FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000860946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28131548
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3765681.3385967953132192Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2010900.7148202438059932Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG842280.299407625915218Illumina Single End PCR Primer 1 (98% over 50bp)
ACTCCAGTCACAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAG440070.15643291297016432Illumina PCR Primer Index 9 (100% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)