FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000860947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30622344
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC15864585.180720326308137TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2317710.7568689059204613TruSeq Adapter, Index 9 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC1101380.3596654782533956TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT756850.24715612887112756TruSeq Adapter, Index 9 (100% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT747470.2440930060742574TruSeq Adapter, Index 9 (100% over 50bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT567210.18522749270924524TruSeq Adapter, Index 9 (100% over 50bp)
TCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAA359890.11752529460187633TruSeq Adapter, Index 9 (100% over 46bp)
CGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAA350560.11447849975168459TruSeq Adapter, Index 9 (100% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)