FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000860981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22489793
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT6800423.0237806101639086TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA2788751.2400069667159677TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT387900.17247824379708607TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT362910.16136653636607504TruSeq Adapter, Index 15 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC234620.10432288105097277TruSeq Adapter, Index 15 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)