FastQCFastQC Report
Tue 26 Sep 2023
EGAF00000861001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34613665
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC11535143.332539330926095TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1245400.3598000962914502TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC624010.18027851139138257TruSeq Adapter, Index 4 (98% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC406120.11732938421863157TruSeq Adapter, Index 4 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTG397120.11472925504999254TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)