FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000861020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28696560
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1669500.5817770492351697Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG398010.13869606670625328Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG333490.11621253557917742Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)