FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000861035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29938603
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC15161045.06404390345134TruSeq Adapter, Index 9 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC1419210.47404015477943307TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1164090.3888257578351268TruSeq Adapter, Index 9 (100% over 49bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT1038010.34671290440639463TruSeq Adapter, Index 9 (100% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT651660.2176654668890195TruSeq Adapter, Index 9 (100% over 50bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT361480.12074043668637445TruSeq Adapter, Index 9 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTG350140.11695268479962143TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)