FastQCFastQC Report
Tue 24 Jan 2023
EGAF00000861330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173519797
Sequences flagged as poor quality0
Sequence length101
%GC21

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAAAAAACACACATCTAAACTCCAATCACAACTACATCTCATATAC7761950.4473235984710148No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6837130.3940259335365635Illumina Single End PCR Primer 1 (100% over 50bp)
AATAATACAACAACCACCAAAATCTACACTCTTTCCCTACACAACACTCT3720110.2143910991320489Illumina Single End PCR Primer 1 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)