FastQCFastQC Report
Fri 6 Jan 2023
EGAF00000861335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167547384
Sequences flagged as poor quality0
Sequence length101
%GC21

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG6061510.36177885057280273TruSeq Adapter, Index 6 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3506940.20931034053029443TruSeq Adapter, Index 6 (100% over 50bp)
TGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT2313300.13806840457741792Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)