FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000861341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174006148
Sequences flagged as poor quality0
Sequence length101
%GC23

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT107585626.1828631480308385TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA5359070.30798164671744815TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACACTGATATATCTCGTAT2232930.12832477620273508TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACTGATATATCTCGTAT2214930.1272903299945471TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACTGATATATCTCGTAT2173980.12493696487091939TruSeq Adapter, Index 25 (97% over 41bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT2090590.12014460546531952TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACTGATATATCTCGTAT2051150.11787801888471205TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACACTGATATATCTCGTAT2048760.1177406674159582TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACTGATATATCTCGTAT1998940.11487755018862895TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGATATATCTCGTAT1957780.11251211652590574TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATACTGATATATCTCGTAT1951020.11212362450549736TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1754800.10084701145157239TruSeq Adapter, Index 25 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)