FastQCFastQC Report
Tue 7 Feb 2023
EGAF00000861343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155790134
Sequences flagged as poor quality0
Sequence length101
%GC25

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1613254710.35530722375526TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA6685330.4291240933138937TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG3687710.2367101115658582TruSeq Adapter, Index 25 (97% over 43bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACACTGATATATCTCGTAT3458750.22201341710123956TruSeq Adapter, Index 25 (97% over 41bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT3452120.2215878445807101TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACTGATATATCTCGTAT3410890.2189413355277042TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACTGATATATCTCGTAT3385340.21730130869519632TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACTGATATATCTCGTAT3180680.20416440491668103TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACACTGATATATCTCGTAT3145910.2019325562682936TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACTGATATATCTCGTAT3083200.19790726927547286TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATACTGATATATCTCGTAT2988950.1918574638365739TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGATATATCTCGTAT2973750.1908817922962952TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACACTGATATATCTCGTAT2728570.17514395359593182TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT2662760.17091968096002794TruSeq Adapter, Index 25 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)