FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000861344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155790134
Sequences flagged as poor quality0
Sequence length101
%GC25

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG97634656.267062457241355Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6242130.4006755652447157Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG5566580.35731274228187004Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGTGGTCGCCG2757020.17697012828809816Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2361080.15155516844218134Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG2102170.13493601590971094Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAACGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2025630.13002299619307087Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAAGGAAAGAGTGTAGATCTCGGTGGTCGCCG1920890.12329984901354536Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGAGAAAGAGTGTAGATCTCGGTGGTCGCCG1915390.12294680996936558Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGAAAAGAGTGTAGATCTCGGTGGTCGCCG1894020.12157509281043433Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph