FastQCFastQC Report
Fri 6 Jan 2023
EGAF00000861345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155938396
Sequences flagged as poor quality0
Sequence length101
%GC25

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1635381110.487353608536539TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA6737370.43205330905160777TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACACTGATATATCTCGTAT3512330.22523830500347075TruSeq Adapter, Index 25 (97% over 41bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG3496940.2242513768065179TruSeq Adapter, Index 25 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACTGATATATCTCGTAT3441340.22068586623143152TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACTGATATATCTCGTAT3434770.22026454600700138TruSeq Adapter, Index 25 (97% over 41bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT3336590.2139684699591241TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACTGATATATCTCGTAT3220670.20653476517739738TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACACTGATATATCTCGTAT3192050.20469942502166047TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACTGATATATCTCGTAT3117740.19993408166132476TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATACTGATATATCTCGTAT3034620.1946037716073468TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGATATATCTCGTAT3021470.19376048987960603TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACACTGATATATCTCGTAT2761010.17705774016041564TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT2704990.17346529587235204TruSeq Adapter, Index 25 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)