FastQCFastQC Report
Sun 25 Dec 2022
EGAF00000861353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172925803
Sequences flagged as poor quality0
Sequence length101
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC15060520.8709238146489914TruSeq Adapter, Index 4 (100% over 50bp)
TAAGTAGAAGATGGTATATGAGATTGGTTAGTGATTGGAGTTTAGATGTG9536930.551504161585417No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG3697280.21380730555289082TruSeq Adapter, Index 4 (100% over 49bp)
AAGTAGAAGATGGTATATGAGATTGGTTAGTGATTGGAGTTTAGATGTGT2342780.13547891404037604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)