FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000861371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123380063
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAAGATGGTATATGAGATGTTTAAGTGATTGGAGTTTAGATGTG51054844.138013772938339No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG37869003.0692965361834834TruSeq Adapter, Index 3 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC18266831.480533366237623TruSeq Adapter, Index 3 (100% over 50bp)
AAGTAGAAGATGGTATATGAGATGTTTAAGTGATTGGAGTTTAGATGTGT1533740.12431019750735578No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)