FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000861401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609961
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG198771.234626180385736Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT128720.7995224729046232Illumina Paired End PCR Primer 2 (100% over 50bp)
CGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG31060.192923927970926Illumina Paired End PCR Primer 2 (100% over 31bp)
CTGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG30590.19000460259596352Illumina Paired End PCR Primer 2 (100% over 31bp)
GCTCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT26810.16652577298456298Illumina Paired End PCR Primer 2 (98% over 50bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT19280.11975445367931273Illumina Paired End PCR Primer 2 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)