FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000861403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110074
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT10270.9330087032360049Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG5500.49966386249250505Illumina Paired End PCR Primer 2 (100% over 31bp)
AAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3150.2861711212457074No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGT2320.2107673019968385Illumina Paired End PCR Primer 2 (98% over 50bp)
TATAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1690.153533077747697No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG1520.13808892199792866Illumina Paired End PCR Primer 2 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1280.11628540799825571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)