FastQCFastQC Report
Fri 27 Jan 2023
EGAF00000861417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134520328
Sequences flagged as poor quality0
Sequence length101
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC7076610.5260624996394597TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG6634000.49315966580158793TruSeq Adapter, Index 1 (100% over 49bp)
ATTAGTATGGTTTGGGGGATTTTATGTTTTAAATGTAGTGAGTTTGTATA2620090.19477279300121839No Hit
ATTGGATGTATTTGTAGGATATTGTGGTTGTTTGATGTAGAATTTAATTT1890120.14050813197541417No Hit
AGTATGGTTTGGGGGATTTTATGTTTTAAATGTAGTGAGTTTGTATAATT1547930.11507034089301357No Hit
GGGGGATTTTATGTTTTAAATGTAGTGAGTTTGTATAATTTTTTAAGAGT1514840.11261048962057243No Hit

[OK]Adapter Content

Adapter graph