FastQCFastQC Report
Fri 10 Feb 2023
EGAF00000861418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134520328
Sequences flagged as poor quality0
Sequence length101
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6983430.5191356655032836Illumina Single End PCR Primer 1 (100% over 50bp)
ACTAAATACATCTACAAAATATCACAACCACTCAACATAAAACTCAATCT2649630.19696874363850794No Hit
ACTAATATAACCCAAAAAATCCTACATTCCAAATACAACAAACTCATATA1916730.1424862716659448No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1781140.1324067541672958Illumina Single End PCR Primer 1 (100% over 50bp)
ATACTAAATACATCTACAAAATATCACAACCACTCAACATAAAACTCAAT1561910.11610958902806125No Hit
AAACCATACTAATACTAAATACATCTACAAAATATCACAACCACTCAACA1532510.11392404573976357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)