FastQCFastQC Report
Mon 26 Dec 2022
EGAF00000861419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228547353
Sequences flagged as poor quality0
Sequence length101
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC8502210.37201087163761637TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG7628870.333798221675313TruSeq Adapter, Index 1 (100% over 49bp)
ATTAGTATGGTTTGGGGGATTTTATGTTTTAAATGTAGTGAGTTTGTATA3852020.16854362780565654No Hit
ATTGGATGTATTTGTAGGATATTGTGGTTGTTTGATGTAGAATTTAATTT3101410.1357009809691386No Hit
GGGGGATTTTATGTTTTAAATGTAGTGAGTTTGTATAATTTTTTAAGAGT2441120.10681025039043003No Hit
AGTATGGTTTGGGGGATTTTATGTTTTAAATGTAGTGAGTTTGTATAATT2368110.10361572640922251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)