FastQCFastQC Report
Fri 30 Dec 2022
EGAF00000861420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228547353
Sequences flagged as poor quality0
Sequence length101
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC7043360.3081794607352114Illumina Single End PCR Primer 1 (100% over 50bp)
ACTAAATACATCTACAAAATATCACAACCACTCAACATAAAACTCAATCT3870670.16935965125791677No Hit
ACTAATATAACCCAAAAAATCCTACATTCCAAATACAACAAACTCATATA3116590.13636517592920885No Hit
AAACCATACTAATACTAAATACATCTACAAAATATCACAACCACTCAACA2433070.10645802579039276No Hit
ATACTAAATACATCTACAAAATATCACAACCACTCAACATAAAACTCAAT2359970.10325956389440223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)