FastQCFastQC Report
Fri 30 Dec 2022
EGAF00000861422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227641417
Sequences flagged as poor quality0
Sequence length101
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6582710.28917013813879044Illumina Single End PCR Primer 1 (100% over 50bp)
ACTAAATACATCTACAAAATATCACAACCACTCAACATAAAACTCAATCT3750270.1647446255353436No Hit
ACTAATATAACCCAAAAAATCCTACATTCCAAATACAACAAACTCATATA3088350.13566731575915292No Hit
AAACCATACTAATACTAAATACATCTACAAAATATCACAACCACTCAACA2372650.10422751849238401No Hit
ATACTAAATACATCTACAAAATATCACAACCACTCAACATAAAACTCAAT2288310.1005225687907223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)