FastQCFastQC Report
Fri 10 May 2024
EGAF00000861839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9966765
Total Bases508.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG9326239.357329083208041RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2942592.952402309074208RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2700692.7096956735711135RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2665542.6744284629967696RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA2168642.1758715089600287RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2026182.0329364643392314RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1919341.9257401975465458RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1851501.857673979470771RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCC1667241.6727995493021057RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1410661.4153639621281329No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1375801.3803877185827096RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCA1246041.2501950231594705RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG1144081.1478950291293113RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT971370.9746091133883462RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA905840.9088605981981114No Hit
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCA872320.8752288229932179RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA871610.8745164554396537RNA PCR Primer, Index 1 (100% over 28bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCA830370.8331389372579769RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA748260.7507551346901428RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA696420.698742269934126RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC659650.6618496573361567RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA607470.6094956588220952RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA602790.604800052976066RNA PCR Primer, Index 1 (100% over 28bp)
ATGACCTATGAATTGACAGACATGGAATTCTCGGGTGCCAAGGAACTCCA596550.5985392451813603RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG535000.5367840016294154RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA522920.5246637198729979RNA PCR Primer, Index 1 (100% over 28bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG508560.5102558352685149RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC506610.5082993328326694RNA PCR Primer, Index 1 (100% over 27bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT502760.5044364946901025RNA PCR Primer, Index 1 (100% over 30bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA427840.42926666777033473RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC425350.42676836465994733RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT388840.39013661905342406RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC365990.3672104238436444RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA365100.3663174560652328RNA PCR Primer, Index 7 (100% over 35bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA352560.3537356404008723RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC342060.34320062728478096RNA PCR Primer, Index 1 (100% over 26bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG333200.3343110828839649RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA327720.3288128093719477RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA320630.3216991671821298RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC305860.30687991539882803RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC303430.3044418123633897RNA PCR Primer, Index 1 (100% over 27bp)
ATGACCTATGAATTGACAGACTGGAATTCTCGGGTGCCAAGGAACTCCAG290750.29171952985748134RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA289290.2902546613670534RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA265820.2667063987161331RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA260490.26135862539148863No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG252200.2530409817026889RNA PCR Primer, Index 30 (100% over 39bp)
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCC246790.24761294161144565RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC239740.24053943280492718No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC235590.2363755942876149RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA235520.23630536086684095RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG234410.2351916594802827RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG232820.23359635749413177RNA PCR Primer, Index 1 (100% over 29bp)
TAATACTGCCTGGTAATGATGACTGGAATTCTCGGGTGCCAAGGAACTCC222860.22360314505258228RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG222410.22315164449046407RNA PCR Primer, Index 1 (100% over 29bp)
TAATACTGCCTGGTAATGATGACTTGGAATTCTCGGGTGCCAAGGAACTC219420.22015167408883426RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG218020.2187470056733554RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG209380.21007819488068594RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG199290.19995454894341344RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC198010.19867028067783277RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC197560.19821878011571456RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG195250.1959010772301745RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC194370.19501814279758778RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC191100.1917372387128622RNA PCR Primer, Index 1 (100% over 26bp)
TGACCTATGAATTGACAGCCAGATGGAATTCTCGGGTGCCAAGGAACTCC185210.1858275980220262RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC180690.18129252570919452RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT167040.1675970086582758RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA166730.16728597493770547No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT163850.16439637134014898RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC162510.16305190299961925RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG162150.16269070254992468No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA156450.1569716954297608RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCA151880.15238645638780488RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA151240.15174432225501455RNA PCR Primer, Index 1 (100% over 22bp)
CTGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTC148600.14909551895725442RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA147680.14817245114136834No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA147060.14755038370022772RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTCC146350.14683801614666345RNA PCR Primer, Index 1 (100% over 27bp)
TGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA144020.14450024656947363RNA PCR Primer, Index 1 (100% over 28bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG138920.13938324019880072RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA134380.13482810119431932RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG127990.1284167932122409RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC127010.12743352532140567RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC126670.12709239156336083RNA PCR Primer, Index 30 (100% over 36bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC120620.12102221733932726RNA PCR Primer, Index 1 (100% over 26bp)
ACTGGACTTGGAGTCAGAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC119310.11970784903627206RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA118430.11882491460368536RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG118190.11858411430388897RNA PCR Primer, Index 1 (100% over 29bp)
ATGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG114710.11509250995684157RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA114700.11508247661101673RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA112570.11294537395032389RNA PCR Primer, Index 7 (100% over 35bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG110740.11110927166437655RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC109590.10995543689451892RNA PCR Primer, Index 1 (100% over 26bp)
TAATACTGCCGGGTAATGATGGATGGAATTCTCGGGTGCCAAGGAACTCC109240.10960426979064922RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG107070.107427033746657RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG106000.10635346574339818RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC105470.10582169841468118RNA PCR Primer, Index 2 (100% over 34bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGGGCCAAGGAACTCCAG105250.10560096480653453RNA PCR Primer, Index 1 (96% over 29bp)
CTTACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG103140.10348392883749141RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT103070.10341369541671747RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph