FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000861848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9206644
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA299518832.5328968948946No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG8532069.267285668914752No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA4068264.418830574963038No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA2860813.107332052808819No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG2543142.7622877565375616No Hit
GCANTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA2085402.265103331898138No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC1664851.8083136482740074No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1392931.512961726335894No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA964251.0473414634040372No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA958731.0413457933205628No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG723930.7863125803495824Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA625190.6790639455593156No Hit
GCANTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG572270.6215837171503537No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG503750.5471592037228767RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCAAG474200.5150628176781898No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT420560.45680054534529624RNA PCR Primer, Index 1 (100% over 30bp)
GCATGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAGG412420.4479591043164045Illumina Small RNA Adapter 2 (100% over 21bp)
CCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA398340.43266580091507834No Hit
GTATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA388630.4221190696631693No Hit
GCAGTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA382700.4156780690119005No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA334410.3632268175026644No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG308010.33455187362517763RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG290860.315924021825977RNA PCR Primer, Index 1 (100% over 29bp)
GTTNCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA259060.28138374851900433No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA242810.263733451624718No Hit
GCAATGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA234860.25509838329797485No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC233440.25355601889244334No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC232100.25210054825623757No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC206870.22469642575513946No Hit
ACATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA201740.21912436279712783No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGTGGAATTCTCGGGTGCCAA198960.21610480431305912No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC184300.20018152108412143RNA PCR Primer, Index 2 (100% over 34bp)
GTTNCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA183600.19942120060252141No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCCA182280.19798745340864707No Hit
GCANTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG179120.19455514952028122No Hit
CTGGACGCGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGG167680.18212934050670365RNA PCR Primer, Index 34 (100% over 38bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC163230.17729587458796062RNA PCR Primer, Index 1 (100% over 33bp)
GCCCGGATAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGG148510.1613074210320286RNA PCR Primer, Index 34 (100% over 38bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC142930.15524658062155983RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG132450.14386349683989083Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA130740.14200614252055363No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA125620.1364449412837077No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG124230.13493516204167338Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA124180.1348808534358448RNA PCR Primer, Index 1 (100% over 28bp)
GCATTAGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA120040.13038410087323893No Hit
CCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG116910.12698438214837024No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG116740.1267997328885531No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA113340.1231067476922101RNA PCR Primer, Index 1 (100% over 23bp)
TCCNTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC113220.12297640703822152No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC113110.12285692810539867No Hit
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG110190.11968530552500997RNA PCR Primer, Index 1 (100% over 29bp)
GCAGTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG106510.11568819213602698No Hit
GTATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG105250.11431961526914693No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAGG100740.10942097902340961Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC98810.10732466683842669RNA PCR Primer, Index 1 (100% over 31bp)
GCANTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA94210.10232827510219794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)