FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000861863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6798620
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA6346539.335026814265248No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA1971042.8991765976036312No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1876192.7596629904304106RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1659512.440951251871703RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG1446952.128299566676767Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1365772.00889298122266No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1280281.8831468739244142RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1077251.5845127393500444No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA989721.4557660231046887No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG894001.3149727444687305RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT844261.241810838081846RNA PCR Primer, Index 3 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA758671.1159176421097223RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG745021.0958400381253843RNA PCR Primer, Index 7 (97% over 36bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC674840.9926132067978501RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG599670.8820466506438072RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG586580.8627927432331854RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT562410.8272414107568888RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC557200.8195780908478485RNA PCR Primer, Index 1 (100% over 27bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT515760.7586245443928327RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT513140.7547708211372308RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC491560.7230290853143726RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG480480.7067316602486975RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA478340.7035839626277097RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT452990.6662969837996534RNA PCR Primer, Index 3 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG451440.6640171093545455RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA421440.6198905071911653RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG405590.5965769523815126RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG404890.5955473316643671No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG374540.5509059191424143RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT360310.5299752008495842RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG327790.48214196410448Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG267140.3929326833975131RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCA251560.3700162680073309No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA234400.3447758515698774RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC218640.32159467656671503No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA218400.321241663749408No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG212910.3131664955535094RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA210400.3094745698391732No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC206490.30372340269054604RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC202710.29816345081796014RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA199330.29319185364088596RNA PCR Primer, Index 1 (100% over 32bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG193320.2843518243408221No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA187100.2752029088256146No Hit
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA183530.26995184316817233RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT181740.2673189559057573RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG177830.2615677887571301No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA172870.2542721905327846No Hit
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCAG170420.25066851802277523RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC169100.2487269475275865RNA PCR Primer, Index 10 (100% over 37bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC168240.24746198493223623RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG164730.24229917247912078Illumina Small RNA Adapter 2 (100% over 21bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC162830.23950448767544003RNA PCR Primer, Index 1 (100% over 31bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT156480.23016435688419118RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA155840.22922298937137242No Hit
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG150050.22070655515384002RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC148570.21852964278044662RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAA145840.21451412198357903No Hit
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCAG143540.21113108248438656RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA139360.20498277591628888RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG138010.20299707881893678No Hit
TTCACCGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC136960.2014526477432185RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA134250.19746654468112645RNA PCR Primer, Index 1 (100% over 22bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG133490.19634867075965418RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGCC131760.1938040367015659No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG130710.1922596056258476RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT130570.1920536814824185RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG129670.19072988341751707RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA129410.19034745286543445RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA127450.18746451485742696RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG124200.18268413295639407RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCCA121750.1790804604463847No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT120530.17728597862507392RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA116160.1708582035766082RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCCA115680.1701521779419941No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA113990.16766637935345702RNA PCR Primer, Index 7 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGGGCCA111450.1639303270369575No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT111210.16357731421965047No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA109870.16160632598968613No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA109670.16131214864193028No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT108790.16001776831180445RNA PCR Primer, Index 1 (100% over 30bp)
ACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC101730.1496333079360223RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG100060.14717692708226082Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC99470.146309103906381No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAATTCTCGGGTGCCAA99220.14594138222168615No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT96040.14126396239236785RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA95880.14102862051416318RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAGG94900.1395871515101594Illumina Small RNA Adapter 2 (100% over 21bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC94770.1393959362341181RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAAG92400.13590993466321108No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT92370.13586580806104767RNA PCR Primer, Index 1 (100% over 30bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCCA92350.1358363903262721No Hit
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA89090.13104129955785146RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG86330.12698165215882046RNA PCR Primer, Index 1 (100% over 29bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGAATTCTCGGGTGCCA84690.12456939790722235No Hit
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA80390.11824458493047119RNA PCR Primer, Index 7 (97% over 35bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC80380.1182298760630834RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG79560.11702374893728432RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA79440.11684724252863082RNA PCR Primer, Index 1 (100% over 32bp)
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG78930.11609709029185335RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA78350.11524397598336132No Hit
TAATACTGCCTGGTAATGATGACTGGAATTCTCGGGTGCCAAGGAACTCCA77290.11368483604025521RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC76480.11249341778184396No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTGGAATTCTCGGGTGCCAA74750.1099487837237557No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC73790.10853673245452752No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC73040.10743356740044302RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC72380.10646278215284866RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA71340.10493305994451814RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC70930.10432999638161863RNA PCR Primer, Index 1 (100% over 31bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG70820.1041681988403529RNA PCR Primer, Index 10 (100% over 45bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG70240.10331508453186088No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA68030.10006442483915853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)