FastQCFastQC Report
Fri 10 May 2024
EGAF00000861872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9729951
Total Bases496.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC214060822.000193012277247No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA192876019.822915860521807No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA5106605.248330644213932No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC4098694.2124467019412535No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2039302.095899557973108RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1693921.7409337415984931RNA PCR Primer, Index 1 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1230671.2648265135148162No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC954990.9814951791638006No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA930290.9561096453620372RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA902830.9278875094026682No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA713240.733035551772049No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT613020.6300340053099959RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC567810.5835692286631248No Hit
GTATCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC482890.49629232459649597No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC449710.46219143344092894RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC449300.46177005413490774No Hit
GTGTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC421530.4332293143100104No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC417870.42946773318796777No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG416980.4285530317675803No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA379640.39017668228750585RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA313450.32214961822520993RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGCATTCTCGGGTGCCAA307200.31572615319439945No Hit
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA296140.3043591894758771RNA PCR Primer, Index 1 (100% over 28bp)
GTCTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC265070.2724268601147118No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA260050.26726753300196476No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC248900.2558080713869988No Hit
GCTTTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA247800.2546775415415761No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC233460.2399395433748844RNA PCR Primer, Index 1 (100% over 27bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC229330.23569491768252482No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA226740.23303303377375695RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG200000.2055508809859371No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG199660.20520144448826103RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCC190750.19604415274033757RNA PCR Primer, Index 1 (100% over 27bp)
GATTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC189390.19464640674963316No Hit
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG173400.1782126138148075RNA PCR Primer, Index 1 (100% over 29bp)
CTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT168360.17303273161396188RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC161860.16635232798191893No Hit
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC147890.1519945989450512RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT147710.15180960315216385RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC145900.14994936767924114RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA144830.14884967046596637No Hit
GTATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA139790.14366978826512075No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA137810.14163483454335998RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG136770.1405659699622331No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC128480.132045885945366No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGC126670.1301856504724433RNA PCR Primer, Index 14 (100% over 50bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC124660.12811986411853463RNA PCR Primer, Index 1 (100% over 27bp)
GGTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC123040.12645490198254852No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA120620.12396773632261868RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTTGGAATTCTCGGGTGCC112410.11552987265814596No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGC110490.11355658420068096No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC108500.1115113529348709No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC106030.10897279955469456RNA PCR Primer, Index 1 (100% over 26bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT105820.10875697112965933RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC105500.10842808972008183RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA101040.10384430507409544No Hit
GCTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC99950.10272405277272209No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCA98550.10128519660582051No Hit
GTTTCCGTAGTGTAGTGGTTATCACCTTCGCCTTGGAATTCTCGGGTGCC97930.10064798887476413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)