FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000861884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9742102
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT6044376.204379711893799RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2516292.5829025399241354RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA1742961.7891005452416737No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1690691.7354468265678187RNA PCR Primer, Index 1 (100% over 31bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1322761.3577767919079478RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1178261.2094515126201717RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG974401.00019482448449RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA931520.956179682783038RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA892380.9160035483102107RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC828200.8501245419109756RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA806550.8279014118308349RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA789360.8102563491944551No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG711290.7301196394782152RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG707260.7259829552184939RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG705690.7243713933604883RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG699560.7180791168066194RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA686290.7044578264526485RNA PCR Primer, Index 3 (100% over 36bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC673100.6909186539003594RNA PCR Primer, Index 1 (100% over 27bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT647140.664271427254611RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT620540.6369672581954079RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA611540.6277290055062039RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG609350.6254810306851643RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCAG609030.6251525594784371RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC600530.6164275430497442RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG581100.5964831819662738RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG565410.5803778281114281RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG564810.5797619445988145RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA492230.5052605690229891RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC447670.45952095348621885RNA PCR Primer, Index 1 (100% over 31bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC407360.418143846163795RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA396690.4071913843644832No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA390710.40105307868876755No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC371170.3809958056279846RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT366180.37587370774808143RNA PCR Primer, Index 3 (100% over 34bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA334640.34349876443502647RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT333320.34214382070727656RNA PCR Primer, Index 3 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA319130.3275781756339648RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA298460.30636098862442623RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA295250.3030660118319435No Hit
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT288020.2956446155049496RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC287980.29560355660410864No Hit
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC279270.2866629809460012RNA PCR Primer, Index 1 (100% over 31bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG260670.2675705920549795RNA PCR Primer, Index 1 (100% over 29bp)
TAATACTGCCTGGTAATGATGACTGGAATTCTCGGGTGCCAAGGAACTCCA254550.2612885802263208RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG246430.25295362335561666RNA PCR Primer, Index 1 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA229010.23507247203940176No Hit
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA223740.2296629618536123RNA PCR Primer, Index 1 (100% over 28bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAAG222310.2281951061485499No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC219010.22480774682917506No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG216430.2221594477249366No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC216280.22200547684678318No Hit
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA215040.22073265092071506RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA210250.21581584754501648No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGGAATTCTCGGGTGCC203850.2092464234104714No Hit
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG203840.20923615868526116RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA201510.20684447771127834RNA PCR Primer, Index 1 (100% over 32bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT195720.20090120181455706RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC194060.19919725742965944RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA189980.19500924954388696No Hit
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG188380.19336689351025066RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT186890.19183744945392686RNA PCR Primer, Index 1 (100% over 30bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC184450.18933285650263157RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG182980.18782394189672824RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG177380.1820756957790013RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG173810.17841118887895036RNA PCR Primer, Index 3 (100% over 37bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC169210.17368941528224607RNA PCR Primer, Index 1 (100% over 27bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT167230.1716569996906212RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA158240.16242901172662738RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA149990.15396061342819034No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGGAATTCTCGGGTGC144570.14839713236424748No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT140000.14370615294317385RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC129720.13315401542706082RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT129180.13259972026570857RNA PCR Primer, Index 1 (97% over 35bp)
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACTCC126850.13020803929172575RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC126530.12987956808499848No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT125800.12913024314465193RNA PCR Primer, Index 1 (100% over 30bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC123970.12725179843118045No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC117980.12110322803025467No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT117620.1207336979226865RNA PCR Primer, Index 1 (100% over 30bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC116960.12005622605881154RNA PCR Primer, Index 1 (100% over 33bp)
AGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG115350.11840360529996503RNA PCR Primer, Index 3 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA113550.11655595476212423RNA PCR Primer, Index 1 (100% over 28bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT113100.11609404212766404RNA PCR Primer, Index 1 (97% over 35bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT112300.11527286411084589RNA PCR Primer, Index 1 (100% over 30bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG112170.11513942268311295RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA112080.1150470401562209No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA111610.11456459807134024No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT102610.10532634538213623RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA102480.10519290395440326RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC101880.10457702044178965No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC101270.10395087220396583No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT99240.10186713298628981RNA PCR Primer, Index 3 (100% over 34bp)
TGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCAG98410.101015160793841RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)