FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000861906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8534492
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT4008734.697092691632964RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1115061.3065335347434857RNA PCR Primer, Index 1 (100% over 29bp)
CTGGACGCGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG1068471.2519432908250427RNA PCR Primer, Index 30 (100% over 38bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG934151.0945584107407915RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA772570.9052325551421221No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG568670.6663196825306064RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA499390.5851432047742268RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG489120.5731096824509297RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA485230.568551707588454RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA424250.4971004718265598No Hit
CTGNCCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT377510.44233447052267433RNA PCR Primer, Index 1 (100% over 30bp)
CTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC362240.42444236868462704RNA PCR Primer, Index 1 (100% over 31bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC348200.4079914774072083RNA PCR Primer, Index 30 (100% over 36bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA300830.3524872950844643RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG295480.34621861500368156No Hit
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG287420.3367745848259041RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC267700.3136683472197291RNA PCR Primer, Index 1 (100% over 27bp)
CACGTCTCGTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC260570.3053140128316952RNA PCR Primer, Index 29 (97% over 37bp)
GCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC260550.3052905785136362RNA PCR Primer, Index 30 (100% over 42bp)
CCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCT259040.3035212875001816RNA PCR Primer, Index 30 (100% over 43bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC255980.2999358368371544RNA PCR Primer, Index 1 (100% over 31bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG250060.2929992786916902RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG244490.28647282111225836RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG242470.2841059549882992RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG227660.26675284246560893RNA PCR Primer, Index 1 (100% over 29bp)
AGCGGTCGGCGTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA203240.2381395401155687RNA PCR Primer, Index 7 (100% over 35bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT201720.2363585319430846RNA PCR Primer, Index 1 (100% over 30bp)
AGCGGTCGGCGTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC197910.2318942943528449RNA PCR Primer, Index 30 (100% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG196650.23041793231512783No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT187080.2192046111238958RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA184060.21566602909698665No Hit
CGCGCGTCCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG177230.2076632094798378RNA PCR Primer, Index 30 (100% over 39bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG176830.2071945231186578RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT174770.20478078835858068RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG169350.1984300881645914RNA PCR Primer, Index 1 (100% over 29bp)
CTGGACGCGAGCCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA168720.19769190714573284RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCG168580.19752786691931987RNA PCR Primer, Index 30 (100% over 45bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG167480.1962389794260748RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC166870.19552423272527525RNA PCR Primer, Index 1 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC165290.19367292159861418RNA PCR Primer, Index 1 (100% over 31bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGT157920.1850373753938723RNA PCR Primer, Index 30 (100% over 46bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC155890.1826587921108837RNA PCR Primer, Index 1 (100% over 27bp)
CTGCCCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT152910.17916707872009252RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA151970.1780656657713195RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC140180.16425113527553836RNA PCR Primer, Index 30 (100% over 44bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCG139240.16314972232676533RNA PCR Primer, Index 30 (100% over 45bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCAG135990.15934164564217765RNA PCR Primer, Index 1 (100% over 29bp)
ATGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT131250.1537877122621944RNA PCR Primer, Index 1 (100% over 30bp)
CGCGAAGCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC129160.1513388260250288RNA PCR Primer, Index 29 (97% over 37bp)
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA127650.14956953501157422RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA126860.14864387944824367No Hit
CTGGACGCGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG126790.14856185933503716RNA PCR Primer, Index 30 (100% over 39bp)
CCCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC118450.13878974870443372RNA PCR Primer, Index 29 (97% over 37bp)
GAGGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC117450.13761803280148366RNA PCR Primer, Index 30 (100% over 42bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC114110.1337045016856305RNA PCR Primer, Index 1 (100% over 27bp)
GCCGGGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT112050.1312907669255534RNA PCR Primer, Index 30 (100% over 41bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC110450.1294160214808333No Hit
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC110110.1290176380738303RNA PCR Primer, Index 30 (100% over 44bp)
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC109930.1288067292112993RNA PCR Primer, Index 1 (100% over 31bp)
GGCGGCGGCGGTCGGCGGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA108970.12768188194446722RNA PCR Primer, Index 1 (100% over 28bp)
CGGGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT108070.1266273376318122RNA PCR Primer, Index 30 (100% over 41bp)
TCTGGACGCGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC107680.12617036842966167RNA PCR Primer, Index 29 (97% over 37bp)
AGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC107090.12547905604692114RNA PCR Primer, Index 29 (97% over 37bp)
CCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG106170.12440107741620708RNA PCR Primer, Index 30 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA98180.11503906735163616RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCG98130.11498048155648866RNA PCR Primer, Index 30 (100% over 45bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGAA97560.11431260349180712RNA PCR Primer, Index 1 (100% over 23bp)
GCGGCGGCGGTCGGCGGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG96190.11270735270476556RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA95520.11192230304978902RNA PCR Primer, Index 1 (100% over 28bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT93270.1092859422681514RNA PCR Primer, Index 1 (100% over 30bp)
CACCCCACGTCTCGTCGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT93260.10927422510912191RNA PCR Primer, Index 1 (100% over 30bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC92860.10880553874794188RNA PCR Primer, Index 2 (100% over 34bp)
CCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG90050.10551301706065222RNA PCR Primer, Index 30 (100% over 39bp)
CGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT87260.10224392969142158RNA PCR Primer, Index 30 (100% over 41bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC86840.10175180901218256No Hit
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC86590.10145888003644506RNA PCR Primer, Index 30 (100% over 44bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC86390.10122453685585504RNA PCR Primer, Index 1 (100% over 27bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT85400.1000645381119345RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)