FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000861907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9333584
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA116650712.49795362638832No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA110861711.8777202840838No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA3837354.111336009832879No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA3279723.5138913412039794No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG2769322.9670488849728036No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1304191.397308900846663No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG1091271.1691864561351781No Hit
GCANTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1005461.0772496395811084No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA937091.003998035481333No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG920890.9866413587749358Illumina Small RNA Adapter 2 (100% over 21bp)
GTTNCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA916480.9819164856715277No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA759270.8134817236337081No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA685750.734712410580973No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA615780.6597465668064915No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA577430.618658384603385No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC500950.5367177281524439No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA475470.5094184613327527No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC461200.4941295862339697No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT433540.4644946678574918RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG375160.4019463477266611No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC368760.3950893890278376RNA PCR Primer, Index 1 (100% over 33bp)
GCANGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA324010.34714424812590744No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC311280.3335053287140288RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCCA277940.29778485949234507No Hit
GTTNCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA267320.2864065936514848No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG267140.28621374168808034Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA248170.2658892875448488No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAA234440.25117896833627895No Hit
GCANTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG233250.24990400257821646No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG227030.24323989584279737RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA222300.23817217480444813No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA219970.23567581327815765RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAAT215540.23092951217881574RNA PCR Primer, Index 46 (100% over 41bp)
GATTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA200880.21522279115932316No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG197970.2121050177509518RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC196060.2100586441392717No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC191460.20513020507449226No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC188580.2020445736600217No Hit
GCAGTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA186850.20019105201174595No Hit
GTTGCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA185840.1991089382170879No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAA185540.19878751827808053No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTTGGAATTCTCGGGTGCCA173890.18630571064662835No Hit
CTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC171090.18330579121589305RNA PCR Primer, Index 1 (100% over 31bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATCTCGT167620.17958803392137468RNA PCR Primer, Index 46 (100% over 46bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAA167300.17924518598643352No Hit
GGTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA166360.17823807017754378No Hit
GCTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA159010.17036328167186368No Hit
CCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA152720.1636241769506762No Hit
CTGGACGCGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCG148550.15915643979847396RNA PCR Primer, Index 46 (100% over 38bp)
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCCA146900.15738863013393356No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG141500.15160307123180122RNA PCR Primer, Index 1 (100% over 29bp)
GTATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA140250.15026382148593725No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG139070.14899956972584164No Hit
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCA137210.14700676610399607No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGGAATTCTCGGGTGCCA135070.14471397053907695No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA133270.14278545090503283No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGGAATTCTCGGGTGCCAA130940.1402890893787424No Hit
CGGGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATC129870.13914269159628284RNA PCR Primer, Index 46 (100% over 42bp)
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG128690.13787843983618728No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG118260.12670373995669829No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC113760.12188244087158802RNA PCR Primer, Index 1 (100% over 31bp)
GCAATGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA113260.12134674097324244No Hit
CTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA112830.12088603906066522No Hit
GTTACCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA106700.11431835830694832No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGAATTCTCGGGTGCCA106210.11379337240656966No Hit
GGGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATCT104940.11243269466477188RNA PCR Primer, Index 46 (100% over 43bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC104060.11148986284368362RNA PCR Primer, Index 5 (97% over 35bp)
GCATGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAGG104050.11147914884571672Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCCA99960.10709712367724981No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCCA99750.10687212971994466No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA98520.10555430797001453No Hit
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA97740.10471861612859541RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)