FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000861912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9215693
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3941414.276846027748537RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1475401.600964789083143RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1333761.4472704331622157RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1015341.101751110849721RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG883990.9592224914610328RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG837830.909134017376664RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG743440.8067109006343853RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGT743290.8065481347957228RNA PCR Primer, Index 37 (100% over 46bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGT682670.7407690338643008RNA PCR Primer, Index 37 (100% over 46bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA607710.6594295187567555RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG575000.6239357148724465RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC501640.544332368710633RNA PCR Primer, Index 1 (100% over 33bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA423570.45961817521482107RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA378970.4112224658525409RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA375580.4075439578987711RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC369940.4014239623650657RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG365000.39606354074511807RNA PCR Primer, Index 1 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG364880.39593332807418824RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT358280.38877163117304364RNA PCR Primer, Index 1 (100% over 30bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT326390.3541676138734222RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG305490.33148890701979766RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA294670.31974806452428484RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCAG287190.31163147470298763RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC283010.3070957333322627RNA PCR Primer, Index 1 (100% over 31bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG270550.29357531766737455RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG254440.2760942665950352RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC251280.27266533292721445RNA PCR Primer, Index 1 (100% over 31bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG248350.2694859735453427RNA PCR Primer, Index 37 (100% over 45bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC245540.26643682683440084RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC237490.2577017268261866RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG236270.25637789800506594RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC233930.2538387509219328RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC228270.2476970532764058RNA PCR Primer, Index 37 (100% over 51bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG217490.23599961500453628RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT217410.2359128065572497RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG215360.23368834009553052RNA PCR Primer, Index 1 (100% over 29bp)
CTGNCCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT215050.23335195736229494RNA PCR Primer, Index 1 (100% over 30bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGT213660.23184366059069025RNA PCR Primer, Index 37 (100% over 46bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG209510.2273404723876978RNA PCR Primer, Index 37 (100% over 45bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT201570.21872473399450262RNA PCR Primer, Index 1 (100% over 30bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGT187240.2031751708742902RNA PCR Primer, Index 37 (100% over 46bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC184450.20014772627517RNA PCR Primer, Index 37 (100% over 44bp)
GCCGGTGATGAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG181920.19740240912973123RNA PCR Primer, Index 7 (97% over 36bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG179540.19481985782295483RNA PCR Primer, Index 7 (97% over 36bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC177380.1924760297462166RNA PCR Primer, Index 1 (100% over 27bp)
ATGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT176670.191705604776548RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC171200.18577007719332667RNA PCR Primer, Index 1 (100% over 31bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG167920.182210930854576RNA PCR Primer, Index 37 (100% over 45bp)
GCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC166890.18109327209576098RNA PCR Primer, Index 37 (100% over 44bp)
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC164510.1785107207889846RNA PCR Primer, Index 1 (100% over 31bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG163580.17750157258927787RNA PCR Primer, Index 37 (100% over 45bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG156400.1697105144453054RNA PCR Primer, Index 1 (100% over 29bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTA156220.16951519543891058RNA PCR Primer, Index 37 (100% over 47bp)
CGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC154200.1673232821449239RNA PCR Primer, Index 37 (100% over 44bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA150670.1634928594084026RNA PCR Primer, Index 1 (100% over 32bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC142830.15498563157431566RNA PCR Primer, Index 37 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG139490.1513613789001001RNA PCR Primer, Index 37 (100% over 45bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA136270.14786733889681436RNA PCR Primer, Index 1 (100% over 28bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATA130890.14202947081679043RNA PCR Primer, Index 37 (100% over 40bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGT127510.13836181391893154RNA PCR Primer, Index 37 (100% over 46bp)
GCCGGTGATGAGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG126560.13733096360740316RNA PCR Primer, Index 2 (100% over 35bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGT124570.13517160348114893RNA PCR Primer, Index 37 (100% over 46bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG122510.13293628596351895RNA PCR Primer, Index 37 (100% over 45bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCT121100.1314062870800926RNA PCR Primer, Index 37 (100% over 43bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA118540.12862841676692138RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT117890.12792309813271774RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA115660.12550331266460374RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG111720.12122799663573862RNA PCR Primer, Index 37 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG108660.11790757352702613RNA PCR Primer, Index 37 (100% over 45bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT108220.11743012706694983RNA PCR Primer, Index 1 (100% over 30bp)
CCTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCT105940.1149560863192817RNA PCR Primer, Index 37 (100% over 43bp)
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA105600.11458715041831363RNA PCR Primer, Index 1 (100% over 28bp)
AGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCT104270.11314395998217389RNA PCR Primer, Index 37 (100% over 43bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT104200.1130680025907981RNA PCR Primer, Index 1 (100% over 30bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC103450.11225417339748621RNA PCR Primer, Index 37 (100% over 44bp)
TGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCAG101580.11022502594216191RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATAT101030.10962821786706653RNA PCR Primer, Index 37 (100% over 41bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG99860.10835864432549999RNA PCR Primer, Index 1 (100% over 29bp)
AGGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCT99210.10765332569129635RNA PCR Primer, Index 37 (100% over 43bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCG97180.10545056134139885RNA PCR Primer, Index 37 (100% over 45bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG93190.10112099003297961RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)