FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000861920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8279321
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT6148097.425838423223354RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG3564254.305002789479959RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2473432.98747928725073RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2461372.97291287534328RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2083632.5166677315688086RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA1690982.0424138646152263RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1575601.9030546103961907RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1456951.7597457569286177RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1345451.6250728773531065RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1320911.5954327655613305RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1271891.5362250116887606RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG897591.0841347980106097RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG871481.0525983954481293RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG778270.9400166994370674RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA760000.9179496724429455RNA PCR Primer, Index 2 (97% over 36bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG756280.9134565503620404RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC723760.8741779670096135RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA715990.8647931394374007RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG604650.7303135124245093RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA599880.7245521704014134RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG582040.7030045096693316RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG565140.6825922077426397RNA PCR Primer, Index 1 (100% over 29bp)
CTGNCCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT513610.6203528042939753RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT450100.5436436152191707RNA PCR Primer, Index 1 (100% over 30bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT448530.5417473244484663RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT387180.4676470449690258RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC340270.4109878092660014RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG328360.3966025716360073RNA PCR Primer, Index 1 (100% over 29bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT296240.3578071196901292RNA PCR Primer, Index 1 (100% over 30bp)
TAGNTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG294820.3560920031968805RNA PCR Primer, Index 1 (100% over 29bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG293990.3550895055282915RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA293560.3545701392662514RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC276740.3342544636208694RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC249920.30186050281176435RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA242280.29263269294668004No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA241470.2916543518484185No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG238240.287753065740536RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG237890.28733032575980566RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG235480.28441945903534843RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC226060.2730417144111214RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA224050.27061397909321305RNA PCR Primer, Index 1 (100% over 28bp)
ATGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT223570.2700342214053544RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG214880.2595381915980791RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG213110.25740033512410015No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC210240.25393386728211165RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG206020.2488368309430206RNA PCR Primer, Index 1 (100% over 29bp)
TGANATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT205560.2482812298254893RNA PCR Primer, Index 1 (100% over 30bp)
TGANATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC204710.24725457558657285RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT203060.24526165853455856RNA PCR Primer, Index 1 (100% over 30bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCAG199140.24052697075037915RNA PCR Primer, Index 1 (100% over 29bp)
CTGCCCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT198440.23968149078891854RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA194060.23439120188720788RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG184680.22306177040363578RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC177470.21435332680059152RNA PCR Primer, Index 1 (100% over 31bp)
TGANATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG176450.21312134171389174RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA170260.20564488319754723RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC165360.19972652346732298RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC149990.18116219917068077RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC148350.17918136040383023RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA144980.17511097830365557RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCA142860.1725503818489463RNA PCR Primer, Index 1 (100% over 28bp)
TAGNTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA142050.17157204075068475RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA137730.16635422155995644RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT135830.16405934737884909RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG132020.15945752073147063Illumina Small RNA Adapter 2 (100% over 21bp)
TAGNTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT128930.1557253306158802RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG128170.15480738094343727RNA PCR Primer, Index 1 (100% over 29bp)
TACNCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT123890.14963787489336383RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA122840.14836965495117294RNA PCR Primer, Index 1 (100% over 32bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG121800.14711351329414574Illumina Small RNA Adapter 2 (100% over 21bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA119520.1443596642768169RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG119400.14421472485485223RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA118190.14275325235004174RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG116380.14056708273540788RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT115040.138948592523469RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC113920.13759582458513206RNA PCR Primer, Index 1 (100% over 27bp)
TACNCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG112030.1353130286891884RNA PCR Primer, Index 1 (100% over 29bp)
TACNCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG111680.1348902887084581RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGGTGCCAAGGAACTCC110280.13319932878553686RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG109640.1324263185350586RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC109140.13182240427687245RNA PCR Primer, Index 1 (100% over 31bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA108870.13149629057745194RNA PCR Primer, Index 1 (100% over 23bp)
CTGGCCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT106070.12811437073160953RNA PCR Primer, Index 1 (100% over 30bp)
TTCNCAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC102110.12333136980677523RNA PCR Primer, Index 1 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT101920.12310188238866449RNA PCR Primer, Index 1 (100% over 30bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT98290.11871746487423306RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG96560.11662792154090898RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT95990.11593945928657676RNA PCR Primer, Index 1 (100% over 30bp)
ACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC91500.11051630924806516RNA PCR Primer, Index 1 (100% over 31bp)
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCCA90360.10913938473940074RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG90200.10894613217678117RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTCC89930.10862001847736064RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA88850.10731556367967855RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT88070.10637345743690818RNA PCR Primer, Index 1 (100% over 30bp)
TAACACTGTCTGGTAACGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT86750.10477912379529675RNA PCR Primer, Index 1 (100% over 30bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG84610.10219437077026003RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA84410.10195280506698556RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC84320.10184410050051206RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph