FastQCFastQC Report
Thu 9 May 2024
EGAF00000861924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9180040
Total Bases468.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA5156555.617132387222713RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4941085.382416634350177RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA4225764.603204343336195RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG2709412.951414155058148RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2645252.8815233920549366RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2582772.8134626864370964No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA2395322.6092696763848524RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1995062.1732585043202426RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1784031.943379331680472RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1672111.8214626515788603RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC1301701.4179676777007508RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC1295671.4113990788711162RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA999231.0884810959429372RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA935461.019015167689901RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC792610.863405823939765RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC790130.8607043106565985No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT607480.6617400359911285RNA PCR Primer, Index 1 (100% over 30bp)
TACNCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA532310.5798558611945046RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA531190.5786358229375907RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC529140.5764027171994893RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC514780.5607600838340574RNA PCR Primer, Index 1 (100% over 27bp)
TGANATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT501150.5459126539753639RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA470580.5126121454808475No Hit
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG465710.5073071577030165RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC449230.4893551662084261RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG442530.4820567230643875RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC432180.4707822623866563No Hit
TACNCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA431000.4694968649374077RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG429200.4675360891673675RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC418490.45586947333562816RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA414680.4517191646223764No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA364110.3966322586829687No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA330440.35995485858449416RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG327280.3565126077882013RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC323900.35283070662001476No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA314610.34271092500686273RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA312980.34093533361510403RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC307990.3354996274526037No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA299350.3260879037564106RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC292730.31887660620215164RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC285710.31122958069899476RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC285440.31093546433348873RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG281760.3069267672036287RNA PCR Primer, Index 1 (100% over 29bp)
TGANATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG272620.29697038357131345RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC267080.2909355514790785No Hit
TAGNTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA266450.29024927995956445RNA PCR Primer, Index 1 (100% over 28bp)
GCANTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA262390.2858266412782515No Hit
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG257810.2808375562633714RNA PCR Primer, Index 1 (100% over 29bp)
TGANATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA241930.26353915669212774RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA214040.2331580254552268RNA PCR Primer, Index 10 (100% over 35bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA205810.22419292290665402RNA PCR Primer, Index 1 (100% over 28bp)
TTCNAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA201540.2195415270521697RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC198110.21580515989037086RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG196930.21451976244112225RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC192720.20993372577897262No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC191470.20857207593866695No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA179300.1953150530934506RNA PCR Primer, Index 1 (100% over 28bp)
CTGNCCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG178960.1949446843368874RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC177110.19292944257323497RNA PCR Primer, Index 1 (100% over 27bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG176300.19204709347671686RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCC176140.19187280229715775RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC173880.18941093938588502RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC169230.18434560197994782RNA PCR Primer, Index 1 (100% over 31bp)
AAGNTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA165000.17973777892035328RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTCC163250.17783146914392528RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA156510.17048945320499695RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC149450.16279885490695029RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCC141460.15409518912771622RNA PCR Primer, Index 1 (100% over 27bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCC140620.15318016043503077RNA PCR Primer, Index 1 (100% over 27bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG140570.15312569444141855RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCA133030.14491222260469452RNA PCR Primer, Index 1 (100% over 28bp)
TACNCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC132900.14477061102130276RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTCC130830.14251571888575648RNA PCR Primer, Index 1 (100% over 27bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG129520.1410887098531161RNA PCR Primer, Index 1 (100% over 29bp)
TAGNTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC127840.13925865246774524RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC125380.1365789255820236RNA PCR Primer, Index 1 (100% over 26bp)
TTCCAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA121080.13189485013137198RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG119690.13038069550895204RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCA118500.129084404860981RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC115260.1255550084749086RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC114440.12466176617966807No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA111520.12148095215271393RNA PCR Primer, Index 1 (100% over 28bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCA110260.12010840911368578RNA PCR Primer, Index 1 (100% over 28bp)
TACACTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA108190.11785351697813952RNA PCR Primer, Index 1 (100% over 28bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC107920.1175594006126335No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA103950.11323480071982257RNA PCR Primer, Index 1 (100% over 28bp)
TACGCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA101300.11034810305837446RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA100700.10969451113502773RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA98620.10742872580075903RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC97350.10604528956300843RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA96500.10511936767160057RNA PCR Primer, Index 1 (100% over 28bp)
ACCNTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA95830.10438952335719671RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG95220.10372503823512752RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA92200.10043529222094894RNA PCR Primer, Index 1 (100% over 28bp)
TGANATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA92160.10039171942605915RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph