FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000861928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10158242
Sequences flagged as poor quality0
Sequence length50-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC6898796.791322750531046No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA4069054.005663578402641RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG3755723.697214537712332RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2663632.622136783116606No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2209592.175169679950527RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC980430.9651571600676574RNA PCR Primer, Index 1 (100% over 27bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC935380.9208089352468665No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA820920.8081319582660071RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA805210.7926666838612428RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA685510.6748313340044468RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG676530.6659912217094257RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT629810.6199990116400063RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA604680.5952604791262109No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA538560.5301704763481713RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA538070.5296881094189329No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA524030.5158668202627974RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA519200.5111120605317337RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC471670.46432246839561414No Hit
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCC464400.4571657182414044RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA448560.4415724689370464RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC437560.4307438235867978RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC416340.4098543822838637RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA389940.3838656334432671No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC368110.36237569453454643RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA335620.33039181385913036No Hit
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA315030.31012255860807414RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA311260.30641128651985255RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG309090.3042750901189399RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA289780.28526589541773073RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA278400.27406316959174626RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG255800.25181522550850827RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC251380.24746407892231748RNA PCR Primer, Index 1 (100% over 26bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCA240210.2364680817802923RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG228320.22476330057897811RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA227150.22361152648263352RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCA216800.21342275563035418RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA212650.20933740306639673RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC210940.20765404092558537RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA191250.18827076574864035RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG189980.18702054942183893RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC186540.18363413669412482RNA PCR Primer, Index 1 (100% over 26bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG186340.18343725223321122RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG183170.1803166335277305No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC182210.17937158811534515RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC177140.17438056703118512No Hit
TAATACTGCCTGGTAATGATGACTGGAATTCTCGGGTGCCAAGGAACTCC168710.16608188700367643RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA168260.1656388969666208RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC167750.1651368415912911RNA PCR Primer, Index 4 (100% over 50bp)
TACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC160160.15766507629961957RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCC153650.15125648709688153RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG152270.1498979843165776RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA149440.14711206919465RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC138010.13586012225343716RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC137820.13567308201556924RNA PCR Primer, Index 1 (100% over 27bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT136460.13433426768135667RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG132480.13041626690917582RNA PCR Primer, Index 1 (100% over 29bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCA129640.12762050756420254RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC124510.12257042114176843RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA121640.11974512912765811No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC121470.11957777733588154No Hit
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCC116710.11489192716613761RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA111930.11018638855030231RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG111720.10997965986634302RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC108350.10666215669994866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)