FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000861954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000861954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7430113
Sequences flagged as poor quality0
Sequence length50-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC6175498.31143483282152RNA PCR Primer, Index 1 (100% over 31bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT4260095.733546717257194RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3008714.049346221248587RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2974804.003707615213927RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2183722.939013175169745RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1479281.9909253062503895RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1370741.8448440824520436RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1091541.4690759077284559RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG984261.3246904858647506RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG978781.3173150933236142RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG945571.2726185994748667RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA930431.2522420587681506RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC874611.177115341314459RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG805481.0840750335829346RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG794411.0691762023000189RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG781701.0520701367529672RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA707230.9518428589174889RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG676840.9109417313034135RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG661220.8899191708120724RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC620350.8349132779003496RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA618410.8323022812708232RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG602380.8107279122134482RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC541900.7293294193506882RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA488790.6578500219310258RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC478840.6444585701455684RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA465660.6267199435594049No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG457680.6159798646400129RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA448560.6037054887321364RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA442190.5951322678403411RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT402280.5414184144978683RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT393350.5293997547547393RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG386810.5205977351892226RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT383320.5159006330051777RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA356110.47927938646424356RNA PCR Primer, Index 1 (100% over 28bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT354090.47656071987061305RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA351990.47373438331287826RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA338760.4559284629991495RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCAG337150.4537616049715529RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG330820.4452422190618097RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT324960.4373553941911785RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG304130.4093208272875527RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA243570.3278146644606886RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA242540.32642841367284725RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA242100.32583622887027425RNA PCR Primer, Index 1 (100% over 32bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA239900.3228753048574093RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT236970.31893189242209374RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA228160.3070747376251209RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG227670.30641525909498285RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA225320.3032524538994225RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC224960.30276793906095373RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC220800.29716910092753634RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG203820.2743161510464242RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG203800.27428923355539814RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT202920.27310486395025213RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT193540.2604805606590371RNA PCR Primer, Index 1 (100% over 30bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT193350.2602248444942896RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA190210.2559987984032006RNA PCR Primer, Index 1 (100% over 28bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG184730.2486234058620643RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA181040.243657128767759RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA180080.2423650891985088RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT176970.2381794193439588RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC171370.23064252185666625RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC156090.2100775587127679RNA PCR Primer, Index 1 (100% over 33bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA155650.20948537391019492RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC154480.20791070068517128RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC153820.2070224234813118RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA149240.2008583180363475RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC146850.19764167785873513RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA135680.182608259120689RNA PCR Primer, Index 1 (100% over 28bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT133970.18030681363796217RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC133780.18005109747321474RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC132630.17850334173921714RNA PCR Primer, Index 1 (100% over 27bp)
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG132330.17809957937382648RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCA129760.1746406817769797RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA126990.17091260926987248No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG124700.16783055654739032RNA PCR Primer, Index 7 (100% over 36bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA119900.16137035870113953RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC117250.15780379114018858RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC114420.15399496616000322RNA PCR Primer, Index 2 (100% over 34bp)
TAATACTGCCTGGTAATGATGACTGGAATTCTCGGGTGCCAAGGAACTCCA114140.1536181212856386RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC111130.14956703888621883RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA109390.14722521716695292RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT109340.1471579234393878RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC108430.14593317759770275No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG107400.14454692680986142RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCC104160.14018629326364215RNA PCR Primer, Index 1 (100% over 27bp)
TGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCAG100330.13503159373215454RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG95380.12836951470320843RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG94730.12749469624486195RNA PCR Primer, Index 1 (100% over 29bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA93860.126323785385229RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT93390.12569122434611693RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCA91680.12338977886339011RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG91680.12338977886339011RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG89600.12059035979668142RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG87690.11801973940369413RNA PCR Primer, Index 1 (100% over 29bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG85340.11485693420813384RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG85040.11445317184274317RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC84640.11391482202222228RNA PCR Primer, Index 1 (100% over 27bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGA83850.1128515811266935RNA PCR Primer, Index 33 (100% over 40bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA81770.11005216205998482RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG81340.10947343600292486RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC81310.10943305976638577RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG78100.10511280245670558RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCA78080.10508588496567953RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG77310.1040495615611768RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA76690.10321511933936942RNA PCR Primer, Index 1 (100% over 28bp)
TTCACCGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC75300.10134435371305928RNA PCR Primer, Index 1 (100% over 31bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA75300.10134435371305928RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph