FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000864576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000864576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490362
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTAT602001.7247494672472368TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTA216950.6215687656466579TruSeq Adapter, Index 1 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTATGCCGTC64450.18465133415960866TruSeq Adapter, Index 1 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTATGCCGTCT51230.14677560665627234TruSeq Adapter, Index 1 (96% over 30bp)
GAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTATGCCGTCTT42290.12116221755794956TruSeq Adapter, Index 1 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)