FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000864577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000864577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490362
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG292110.8369045961421767Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC216930.6215114649999054Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATTTCCTGCTGTGACTGGAGTTCAGACG69190.19823158743992744RNA PCR Primer, Index 19 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)