FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000864665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000864665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239738
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3550.14807831883139094Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2530.10553187229392087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)