FastQCFastQC Report
Wed 8 May 2024
EGAF00000864678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000864678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6845187
Total Bases520.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACGCAAATCTCGTAT626460.91518317907166TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACGCAAATCTCGTA447910.6543429712000564TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)