FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000867533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000867533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40593584
Sequences flagged as poor quality0
Sequence length51
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAA25603936.307383452518013Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAA14887083.6673480222884485Illumina PCR Primer Index 4 (95% over 22bp)
TTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAA10860092.6753217946954377Illumina Single End Adapter 1 (95% over 22bp)
AGTTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAA4784731.1786911941552143Illumina Single End Adapter 1 (95% over 22bp)
TCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAA4210221.0371639025516939Illumina Single End Adapter 1 (95% over 22bp)
CTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3940380.9706903435774482Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAA3733550.9197389419963509Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAA3192900.7865528700299043Illumina PCR Primer Index 4 (95% over 22bp)
CAGAGTTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAA2765750.6813268816076945Illumina DpnII expression Sequencing Primer (100% over 23bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAA2325970.5729895640650995Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAG2309170.5688509790118557Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAGA2075570.5113049392238931Illumina PCR Primer Index 4 (95% over 22bp)
ACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1958610.4824925042341667Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGAA1838070.45279815647714183Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACA1779950.43848062294770523Illumina PCR Primer Index 4 (95% over 22bp)
TCAGAGTTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGA1609190.39641486201366205Illumina DpnII expression Sequencing Primer (100% over 24bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAAA1580020.38922899737061895Illumina PCR Primer Index 4 (95% over 22bp)
AGAGTTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAA1550700.38200618107531475Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGAAAAAA1549890.3818066421531048Illumina PCR Primer Index 4 (95% over 22bp)
CAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1490780.3672452277187449Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAC1489890.36702598124866237Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACAA1460730.35984258004910336Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCAA1407920.3468331350097099Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCAAA1251650.30833690368408956Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAAAAA1151040.2835521987908237Illumina PCR Primer Index 4 (95% over 22bp)
ATCACTCGGCTCGTGCGTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAA1118920.27563961831997885Illumina Single End Adapter 2 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAAAAAAA1109210.27324761469694325Illumina PCR Primer Index 4 (95% over 22bp)
CATTTCCACCGCGGCGGTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAA1093700.2694268138531449Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAATGAAAAAA992350.24445981414205753Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCAA932540.2297259586638125Illumina PCR Primer Index 4 (95% over 22bp)
GAGTTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAA908860.22389252449352587Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGAAAA862230.21240548752729005Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAACAA773290.19049562117993818Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAG747540.18415225420844833Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAGG732270.18039057600826772Illumina PCR Primer Index 4 (95% over 22bp)
CGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA728780.179530834232326Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGAG708410.17451279985526777Illumina PCR Primer Index 4 (95% over 22bp)
TAGAGTTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAA698620.17210108868435958Illumina DpnII expression Sequencing Primer (95% over 23bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACAAA694530.1710935402993734Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAG689310.16980762280068692Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACACA684250.1685611203977456Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAC669860.1650162252241635Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAGA644140.15868024858312585Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAC643790.15859402806118328Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAGAAAAA635050.15644097845610283Illumina PCR Primer Index 4 (95% over 22bp)
AACAATGTAGGTAAGGGAAGTAACGTCGTATGCCGTCTTCTGCTTGAAAAA630630.15535213643614224Illumina Single End Adapter 1 (95% over 22bp)
TAGTTCTACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAA617080.1520141705152223Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAACA609900.15024541809365735Illumina PCR Primer Index 4 (95% over 22bp)
GATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA596330.1469025252857693Illumina Paired End PCR Primer 2 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAAG586250.14441937425382298Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACCAA575610.1417982703867685Illumina PCR Primer Index 4 (95% over 22bp)
TACAGTCCGACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA560300.13802673841265162Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCAA557440.13732219357620654Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAGA553670.13639347538271074Illumina PCR Primer Index 4 (95% over 22bp)
CCCGGGTGGAGCCGCCGCAGTAACGTCGTATGCCGTCTTCTGCTTGAAAAA544910.13423549889066214Illumina Single End Adapter 2 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACGAA538570.13267367572175937Illumina PCR Primer Index 4 (95% over 22bp)
CCGGGGAGGTGGAGCACGTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAA532250.13111677943982478Illumina Single End Adapter 2 (95% over 22bp)
AATACCGGGTGCTGTAGGCTTTAACGTCGTATGCCGTCTTCTGCTTGAAAA515570.1270077557083898Illumina Single End Adapter 2 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGGAA509610.12553954339188184Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACCA508340.12522668606940446Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGGA507880.12511336766913705Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCACAA506780.12484238888588897Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACGA505480.12452214123295938Illumina PCR Primer Index 4 (95% over 22bp)
ATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAA493690.12161774136523644Illumina Single End Adapter 1 (95% over 22bp)
ACCGGCTCCGGGACGGCTGTAACGTCGTATGCCGTCTTCTGCTTGAAAAAA489120.12049194769301473Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCCC483480.11910256556799716Illumina PCR Primer Index 4 (95% over 22bp)
GACGATCTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA475930.11724266573752147Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACG471290.11609962796091126Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAG469940.11576706309056131Illumina PCR Primer Index 4 (95% over 22bp)
AACTGGCGCTGCGGGATGTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAA468460.11540247345491839Illumina Single End Adapter 1 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGCAA465400.11464865974879182Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACAC458310.11290207831858355Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAAAAAA454930.11206943442096662Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAGA447540.11024894968623612Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCACA443270.10919705931853663Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAC440140.10842600150802156Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCAAAA439610.10829543900336565Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCAC435750.1073445498185132Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCAC429480.10579997075399895Illumina PCR Primer Index 4 (95% over 22bp)
CCGGGGAGGTGGAGTAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA429280.1057507018843175Illumina Paired End PCR Primer 2 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACCA428310.10551174786636232Illumina PCR Primer Index 4 (95% over 22bp)
TAACGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATTGAAAAAA424440.10455839523802579Illumina PCR Primer Index 4 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)