FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000867534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000867534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39759235
Sequences flagged as poor quality0
Sequence length51
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAA38144569.593886803908577Illumina Single End Adapter 2 (95% over 22bp)
TTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAA18057634.541744829848965Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAA14353303.610054368500802Illumina Single End Adapter 1 (95% over 22bp)
AGTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAA7806771.9635111188633283Illumina Single End Adapter 2 (95% over 22bp)
TCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAA6284111.5805409736882512Illumina Single End Adapter 2 (95% over 22bp)
CTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5649051.420814560441115Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAA3519560.8852182392342308Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAA3031080.7623587325057939Illumina Single End Adapter 1 (95% over 22bp)
ACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2590370.6515140444729381Illumina Single End Adapter 2 (95% over 22bp)
CAGAGTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAA2586590.650563321955264Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2324270.5845861973953975Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAG2279060.5732152542673419Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAA2210830.5560544613094291Illumina Single End Adapter 1 (95% over 22bp)
AGAGTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAA2170790.5459838450111024Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAGA2048340.5151859687441169Illumina Single End Adapter 1 (95% over 22bp)
TAGTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAA1815630.4566561705726984Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGAA1812730.455926780281361Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACA1676880.42175861784060986Illumina Single End Adapter 1 (95% over 22bp)
TCAGAGTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGA1600270.40249013845462567Illumina DpnII expression Sequencing Primer (100% over 24bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAAA1545340.3886744802811221Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGAAAAAA1404350.35321353642744885Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAC1392790.3503060358178421Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACAA1360480.34217962191676976Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCAA1315990.33098976879208064Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCAAA1148260.28880334342448993Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAAAAA1066890.26833765790513825Illumina Single End Adapter 1 (95% over 22bp)
GAGTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAA1034860.2602816678942641Illumina Single End Adapter 2 (95% over 22bp)
TACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1013700.25495963390643706Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAAAAAAA1008870.25374482180052005Illumina Single End Adapter 1 (95% over 22bp)
ACCGGCTCCGGGACGGCTGTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAA896950.2255953868327698Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCAA851540.2141741409260012Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAATGAAAAAA848510.21341205382850048Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGAAAA835570.2101574640457745Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAA767540.19304697386657463Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA745270.18744575945689096Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAGG730480.18372586897107052Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAG730020.1836101725800308Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAACAA717820.18054170307854264Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGAG714540.17971673750765074Illumina Single End Adapter 1 (95% over 22bp)
CTACCCACCCGACCCGTCTTGTAGAGTCGTATGCCGTCTTCTGCTTGAAAA703630.17697272092886093Illumina Single End Adapter 2 (95% over 22bp)
CATTTCCACCGCGGCGGTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAA683340.17186950402843515Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAG665420.1673623750557575Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACACA633340.15929380935020507Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACAAA632310.15903475003983353Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAGAAAAA623880.15691448791708393Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAGA620870.15615743109745447Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAC619760.15587825067559777Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAC596470.15002049209447818Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAAG581730.14631317730333596Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAACA567040.14261843820687195Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAGA556290.13991466385105247Illumina Single End Adapter 1 (95% over 22bp)
CGGGTGTTGACGCGATGTGTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAA535750.13474856847723554Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACCAA531350.1336419073455513Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACGAA513680.12919765684626477Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGGA503700.12668754818849004Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCAA500380.12585252206185557Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGGAA499920.12573682567081584Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACGA480750.12091530433118243Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACCA472300.11879001193056155Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCACAA458380.11528893853214228Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACG448830.11288698084860034Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAG448730.11286182945924386Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGCAA447380.11252228570293166Illumina Single End Adapter 1 (95% over 22bp)
ATTCAACCCGGCGGCGGTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAA445150.1119614097202826Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAGA434280.10922745369723538Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCCC426720.10732600866188698Illumina Single End Adapter 1 (95% over 22bp)
TTTCTACAGTCCGACGATCTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAA424710.10682046573582213Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACAC419250.10544719987695941Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAAAAAA419180.10542959390440988Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCACA410210.1031735142791354Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAC406900.10234100329143657Illumina Single End Adapter 1 (95% over 22bp)
GTTCTACAGTCCGACGACTAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAA406660.10228063995698106Illumina Single End Adapter 2 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCAAAA400030.10061310284264775Illumina Single End Adapter 1 (95% over 22bp)
TAGAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCAC399290.10042698256140996Illumina Single End Adapter 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)