FastQCFastQC Report
Fri 31 Mar 2023
EGAF00000867535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000867535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38833193
Sequences flagged as poor quality0
Sequence length51
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAA18729324.823018287473811Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAA13959023.5946104148582374Illumina PCR Primer Index 2 (95% over 22bp)
TTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAA7926402.0411404233486543Illumina Single End Adapter 1 (95% over 22bp)
CTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3670400.9451707975700065Illumina Single End Adapter 1 (95% over 22bp)
AGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAA3569230.9191183429083465Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAA3495400.9001062570363452Illumina PCR Primer Index 2 (95% over 22bp)
TCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAA3376090.8693825408587957Illumina Single End Adapter 1 (95% over 22bp)
CGTACGCGGGTTTAAACGATTAAGTCGTATGCCGTCTTCTGCTTGAAAAAA3137420.8079222329206872Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAA3050820.7856217231480296Illumina PCR Primer Index 2 (95% over 22bp)
CGTACGCGGAATAGTTTAAACTGTTTAAGTCGTATGCCGTCTTCTGCTTGA2566310.6608547486682333Illumina Single End Adapter 2 (95% over 22bp)
CAGAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAA2457020.6327112993258113Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAG2254290.5805059604550159Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAA2198480.5661342346996808Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAGA1999640.5149306161870336Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGAA1753860.4516394003449575Illumina PCR Primer Index 2 (95% over 22bp)
ACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1742390.4486857416025512Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACA1697760.43719299620816654Illumina PCR Primer Index 2 (95% over 22bp)
CGGGTGTTGACGCGATGTGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAA1645510.42373801196311617Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAAA1511710.3892829518293796Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGAAAAAA1478460.3807206891279839Illumina PCR Primer Index 2 (95% over 22bp)
TCAGAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGA1461050.376237411124035Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1446340.37244941460260556Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAC1427220.3675257916597278Illumina PCR Primer Index 2 (95% over 22bp)
AGAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAA1399950.36050344868628237Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACAA1377660.35476351378059484Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCAA1329130.34226647291146006Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAAAAAAA1200110.3090423185134429Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCAAA1173470.3021822078859186Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAAAAA1107480.2851890134298254Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAATGAAAAAA961180.24751505754368433Illumina PCR Primer Index 2 (95% over 22bp)
TAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAA899140.23153903414535087Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCAA875550.22546433408141328Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGAAAA816560.2102737212466665Illumina PCR Primer Index 2 (95% over 22bp)
AAACTAACCAGGATTCCCTCAGTTAAGTCGTATGCCGTCTTCTGCTTGAAA804700.2072196329567852Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAACAA763310.19656122534142378Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAGG747150.19239983691271537Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAG737070.18980411937797648Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGAG725230.18675518132129904Illumina PCR Primer Index 2 (95% over 22bp)
AACAATGTAGGTAAGGGAAGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAA711010.1830933655133638Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAG691590.17809248907242833Illumina PCR Primer Index 2 (95% over 22bp)
TAGAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAA680570.1752547105771086Illumina DpnII expression Sequencing Primer (95% over 23bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACAAA665570.17139203567422334Illumina PCR Primer Index 2 (95% over 22bp)
GAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAA658620.1696023296358865Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACACA655320.16875254115725175Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAC645880.16632163108503595Illumina PCR Primer Index 2 (95% over 22bp)
CATTTCCACCGCGGCGGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAA635600.16367441121825857Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAGA625170.16098856460245234Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAC625110.1609731139028408Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAGAAAAA620920.15989414004663485Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAAG600700.15468725427754548Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAACA594170.15300570313648945Illumina PCR Primer Index 2 (95% over 22bp)
TACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA587690.151337027578443Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAGA566610.14590868178158822Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACCAA558410.14379708616801096Illumina PCR Primer Index 2 (95% over 22bp)
TAAACGGGTGGGGTCCGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAA549190.14142282866103748Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCAA527460.13582710028505768Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGGA514420.13246914823614944Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACGAA511370.13168373767256275Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGGAA499380.12859617286685646Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACGA496740.12791634208394867Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACCA494260.1272777131666716Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCCC485140.12492920682571737Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCACAA479580.12349744199504789Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAG472480.1216691092076822Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACG471250.1213523698656456Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGCAA452710.11657810368567942Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACAC450800.11608625641471203Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAGA449030.11563046077617156Illumina PCR Primer Index 2 (95% over 22bp)
CGCGGAATAGTTTAAACTGTTTAAGTCGTATGCCGTCTTCTGCTTGAAAAA447310.11518754072064072Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAAAAAA440760.1135008393463808Illumina PCR Primer Index 2 (95% over 22bp)
CTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAA440240.1133669332830808Illumina Single End Adapter 1 (95% over 22bp)
AGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA439360.11314032302211152Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCACA435250.11208195009872095Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAC428650.11038237314145144Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCAC426080.10972056817475709Illumina PCR Primer Index 2 (95% over 22bp)
CTTTGGAAAAGCTGCTAATAAGCCCTCTTAAGTCGTATGCCGTCTTCTGCT423280.10899953552621852No Hit
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACCA419650.10806476819972027Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCAC417100.10740811346622978Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCAAAA416430.1072355806539009Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATTGAAAAAA413880.1065789259204104Illumina PCR Primer Index 2 (95% over 22bp)
CCGGGGAGGTGGAGCACGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAA399780.10294801151169825Illumina Single End Adapter 2 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)