FastQCFastQC Report
Sat 11 May 2024
EGAF00000867535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000867535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38833193
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAA18732814.823917003167883Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAA17960994.625164353598222Illumina PCR Primer Index 2 (95% over 22bp)
TTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAA7927832.0415086650227297Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAA4995421.286378897558076Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACA4476231.1526814187028092Illumina PCR Primer Index 2 (95% over 22bp)
CTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAA3671070.9453433303823356Illumina Single End Adapter 1 (95% over 22bp)
AGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAA3569860.9192805752542675Illumina Single End Adapter 1 (95% over 22bp)
TCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAA3376740.8695499234379208Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAG3246130.8359163255001977Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAA3171250.8166338523849945Illumina PCR Primer Index 2 (95% over 22bp)
CGTACGCGGGTTTAAACGATTAAGTCGTATGCCGTCTTCTGCTTGAAAAA3138030.8080793150334045Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGA2898580.746418148000346Illumina PCR Primer Index 2 (95% over 22bp)
CGTACGCGGAATAGTTTAAACTGTTTAAGTCGTATGCCGTCTTCTGCTTG2566800.6609809293817276Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAC2566710.6609577533323103Illumina PCR Primer Index 2 (95% over 22bp)
CAGAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGA2457670.6328786819049363Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAA2455520.6323250318355228Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACA2242560.5774853486809596Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCA2181480.5617565364764108Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCAA1824070.46971929400706247Illumina PCR Primer Index 2 (95% over 22bp)
ACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1753980.45167030174418055Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGAAAAA1722000.4434350788512291Illumina PCR Primer Index 2 (95% over 22bp)
CGGGTGTTGACGCGATGTGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAA1645870.4238307161607854Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCA1607080.41384183886192416Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAAAA1589000.40918602804564636Illumina PCR Primer Index 2 (95% over 22bp)
CAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1475670.38000223159604724Illumina Single End Adapter 1 (95% over 22bp)
TCAGAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTG1461430.37633526555490815Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAA1400280.3605884275341459Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAAAAAA1354090.34869396394986113Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGAAA1319210.3397119572423519Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAG1216300.313211432291957Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAACA1200980.3092663536578102Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAC1151980.296648282308385Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCC1090720.28087311800500153Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCA1088780.2803735453842284Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAG1081430.2784808346818146Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACAA1035290.26659924668053947Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACCA1005660.25896917618904014Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAATGAAAAA1004890.258770892210692Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAC996360.25657431774925127Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGG993880.25593568883197426Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACG987990.25441894515344127Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACGA974860.25103781705511574Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCC967240.24907557820444998Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGGA946650.24377341312108947Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACC926300.23853305083617513Illumina PCR Primer Index 2 (95% over 22bp)
TAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAA899330.2315879613607874Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAGAAAA894820.2304265837733199Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACC886200.22820683326246183Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCACA858710.22112783772377406Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCAC844350.21742997028341193Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGCA828970.21346944094965356Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAG814650.20978187397569908Illumina PCR Primer Index 2 (95% over 22bp)
AAACTAACCAGGATTCCCTCAGTTAAGTCGTATGCCGTCTTCTGCTTGAA804950.2072840108718333Illumina Single End Adapter 1 (95% over 22bp)
AACAATGTAGGTAAGGGAAGTTAAGTCGTATGCCGTCTTCTGCTTGAAAA711190.1831397176121984Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAT690890.17791223091029368Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGAGA684670.17631050838389725Illumina PCR Primer Index 2 (95% over 22bp)
TAGAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGA680840.17532423872536054Illumina DpnII expression Sequencing Primer (95% over 23bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAGA672600.17320234264537557Illumina PCR Primer Index 2 (95% over 22bp)
GAGTTCTACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAA658690.16962035545209994Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCAAA640970.1650572488334915Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCAA636610.16393449799505283Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAAAAA636510.16390874682903359Illumina PCR Primer Index 2 (95% over 22bp)
CATTTCCACCGCGGCGGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAA635700.1637001623842778Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCG631700.1626701157435084Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCG631160.16253105944700452Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCC622990.160427189183233Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACG622060.16018770333925414Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGAAG589610.1518314499660123Illumina PCR Primer Index 2 (95% over 22bp)
TACAGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAA587830.15137307921086995Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGC582580.1500211429948601Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCACC565160.14553528987430933Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACCCCCC559510.14408034899422256Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAC554310.14274128836122232Illumina PCR Primer Index 2 (95% over 22bp)
TAAACGGGTGGGGTCCGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAA549290.14144857982705672Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCAG547530.14099535930511817Illumina PCR Primer Index 2 (95% over 22bp)
CTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAA529280.13629577150660777Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCG516130.13290949317507833Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACGAA513880.13233009193964557Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAATA512570.13199275166479357Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCAC503130.12956184159257778Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGGG488260.1257326432055175Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGACA478210.12314465102058438Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCGA470000.12103048029040518Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGGAA466360.120093137847305Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAAAC463310.11930772728371834Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACGG461260.11877982838032401Illumina PCR Primer Index 2 (95% over 22bp)
AGTCCGACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA460620.1186150209178009Illumina Single End Adapter 1 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACCC453090.1166759581165525Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACGCA452660.11656522810266978Illumina PCR Primer Index 2 (95% over 22bp)
CGCGGAATAGTTTAAACTGTTTAAGTCGTATGCCGTCTTCTGCTTGAAAA447410.11521329188665995Illumina Single End Adapter 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGCAA445070.11461071460180984Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGGGA431210.11104160299154385Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACAC427820.11016863846349179Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATTGAAAAA425230.1095016832635936Illumina PCR Primer Index 2 (95% over 22bp)
CTTTGGAAAAGCTGCTAATAAGCCCTCTTAAGTCGTATGCCGTCTTCTGC423280.10899953552621852No Hit
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAATAA417690.10756004534574327Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAACC412410.10620038377992765Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGAACAA410750.10577291442400835Illumina PCR Primer Index 2 (95% over 22bp)
TTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACCCCCA406340.10463728800256009Illumina PCR Primer Index 2 (95% over 22bp)
GACGATCTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA402560.10366389392703299Illumina Single End Adapter 1 (95% over 22bp)
CCGGGGAGGTGGAGCACGTTAAGTCGTATGCCGTCTTCTGCTTGAAAAAA399870.10297118756111556Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)