FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000867536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000867536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23502107
Sequences flagged as poor quality0
Sequence length51
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAA10110054.301763241908481TruSeq Adapter, Index 13 (95% over 24bp)
GTTCTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAAAAA4564181.942030133723755TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAA2406931.024133708522389TruSeq Adapter, Index 13 (95% over 24bp)
TTCTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAA2042810.8692029187000128TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAA2041960.8688412489995045TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAA1486190.6323645790566778TruSeq Adapter, Index 13 (95% over 24bp)
CTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAA1412840.6011546113716528TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAG1384750.5892024915042724TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAGA1257390.5350116055551956TruSeq Adapter, Index 13 (95% over 24bp)
CAGAGTTCTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAA1161470.49419824358726644TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACA1134510.4827269316746792TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGAA1126690.4793995704300044TruSeq Adapter, Index 13 (95% over 24bp)
TCTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAA1050360.4469216313243744TruSeq Adapter, Index 16 (96% over 25bp)
AGTTCTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAAAA953280.40561469658869315TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAAA948210.40345744319860344TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAC943700.4015384663170838TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGAAAAAA932160.39662826826547937TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAAAAAAA908850.38671000859625054TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACAA894390.3805573687499593TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCAA858250.36518002407188427TruSeq Adapter, Index 13 (95% over 24bp)
ACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA854550.3636056971402607TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCAAA749620.31895863634694543TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAAAAA693630.2951352404275923TruSeq Adapter, Index 13 (95% over 24bp)
TCAGAGTTCTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGA647410.2754689185952562Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACAATGTAGGTAAGGGAAGTCCTATCGTATGCCGTCTTCTGCTTGAAAAA625060.2659591329407189TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAATGAAAAAA616090.26214245386594487TruSeq Adapter, Index 13 (95% over 24bp)
CCGGGGAGGTGGAGCACGTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAA589110.25066263207805156Illumina PCR Primer Index 1 (95% over 23bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCAA539120.22939219875052053TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAGAAAA528370.22481814077350598TruSeq Adapter, Index 13 (95% over 24bp)
CGGGTGTTGACGCGATGTGTCCTATCGTATGCCGTCTTCTGCTTGAAAAAA493460.20996415342675445TruSeq Adapter, Index 13 (95% over 24bp)
ATCACTCGGCTCGTGCGTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAA487140.2072750328300352TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACAACAA460560.19596540854826336TruSeq Adapter, Index 13 (95% over 24bp)
AAACTAACCAGGATTCCCTCAGTCCTATCGTATGCCGTCTTCTGCTTGAAA435630.1853578489792426TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAG435550.1853238094780183TruSeq Adapter, Index 13 (95% over 24bp)
TCAGGGATCACTCTTGAGAAGAGCCTTTCCTATCGTATGCCGTCTTCTGCT432350.1839622294290465TruSeq Adapter, Index 13 (95% over 22bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACACA421410.17930732763662424TruSeq Adapter, Index 13 (95% over 24bp)
CATTTCCACCGCGGCGGTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAA416710.17730750693969693TruSeq Adapter, Index 13 (95% over 24bp)
CACGGGGTCGGCGGCGACGTCCTATCGTATGCCGTCTTCTGCTTGAAAAAA407860.17354188711675936TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACAAA406280.17286960696757953TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAAC405040.17234199469860298TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAGG400840.17055492088432753TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAGAAAAA395140.16812960642209654TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAG388220.16518518956619507TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGAG387080.16470012667374886TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACAGA384010.16339386081426657TruSeq Adapter, Index 13 (95% over 24bp)
CAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA380590.16193867213692797TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACAC378830.1611898031099935TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAACA360590.15342879683085436TruSeq Adapter, Index 13 (95% over 24bp)
AGAGTTCTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAA358850.15268843767922596TruSeq Adapter, Index 13 (95% over 24bp)
CTTTGGAAAAGCTGCTAATAAGCCCTCTCCTATCGTATGCCGTCTTCTGCT341420.1452720813499828TruSeq Adapter, Index 13 (95% over 22bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACACCAA329650.14026401973235847TruSeq Adapter, Index 13 (95% over 24bp)
AATACCGGGTGCTGTAGGCTTTTCCTATCGTATGCCGTCTTCTGCTTGAAA326260.138821595867979TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACGAA321460.13677922579452131TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAAG315520.13425179282861746TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACCA311130.1323838751989343TruSeq Adapter, Index 13 (95% over 24bp)
CAGGGCGCCCTGGAATGGGTTCGTCCTATCGTATGCCGTCTTCTGCTTGAA310230.13200093081016098TruSeq Adapter, Index 16 (96% over 25bp)
TCTAACACGTGCGCGAGTCGGGGGCTCGTCCTATCGTATGCCGTCTTCTGC305360.12992877617313206TruSeq Adapter, Index 16 (95% over 22bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGAGA304000.12935010465231905TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCAA301580.12832040974028414TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCACAA293290.12479306642591663TruSeq Adapter, Index 13 (95% over 24bp)
ACCGGCTCCGGGACGGCTGTCCTATCGTATGCCGTCTTCTGCTTGAAAAAA286830.12204437670205485TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAACGA285800.12160611812379206TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGGAA284130.1208955435357349TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAGGA275950.11741500453555079TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACG273870.11652997750371914TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATGAAAAAAA268220.11412593772975334TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAAAAAA266660.11346216745587959TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAGCAA266350.11333026438863544TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAG266270.11329622488741116TruSeq Adapter, Index 13 (95% over 24bp)
CAGGGCGCCCTGGAATGTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAA266170.1132536755108808TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAAC264030.1123431188531309TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACAC262910.11186656583599078TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATTGAAAAAA259190.1102837290290611TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCAAAA258500.10999013833100156TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACAAGA255260.10861153853141763TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCACA250170.10644577526602189TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAACCAC247970.1055096889823538TruSeq Adapter, Index 13 (95% over 24bp)
CATCGCGAAGGCCCGCGTCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAA245580.104492758883278TruSeq Adapter, Index 16 (96% over 25bp)
GGTCGCGCCGTCGGGCCCGGGGGTCCTATCGTATGCCGTCTTCTGCTTGAA244600.10407577499328038TruSeq Adapter, Index 16 (96% over 25bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACACCA243860.10376090960695566TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAACCCCCC241090.10258229187706447TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAATA239720.10199936541859843TruSeq Adapter, Index 13 (95% over 24bp)
TCCTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAACCCAC236400.10058672611779021TruSeq Adapter, Index 13 (95% over 24bp)
TAGTTCTACAGTCCGACGATCTCCTATCGTATGCCGTCTTCTGCTTGAAAA235330.10013144778891526TruSeq Adapter, Index 13 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)