FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000884597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000884597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18874975
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTA839810.4449330396464102Illumina Paired End PCR Primer 2 (97% over 42bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG552680.2928109838556077Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG234600.12429155535305343Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)