FastQCFastQC Report
Sun 14 May 2023
EGAF00000884696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000884696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29851997
Sequences flagged as poor quality0
Sequence length126
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT6151602.0606996577146917Illumina Single End PCR Primer 1 (100% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1219880.40864267807611Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT481510.16129909164870948Illumina Paired End Adapter 2 (100% over 30bp)
CGGCATTCCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA299160.10021440106670251Illumina Single End PCR Primer 1 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)