FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134291
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA38992.903396355675362No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA33142.4677752045930106No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT32782.440967749141789No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA32362.4096923844486975No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG31212.3240574573128505No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG30922.302462562643811No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA30602.278633713353836No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT30452.26746394024916No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT30332.25852812176542No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA29162.17140389154895No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG26691.9874749610919569No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA24651.8355660468683679No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC23751.7685474082403139No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT23611.75812228667595No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC23471.747697165111586No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC23211.7283362250634815No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT21901.6307868732826474No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA20801.548875203848359No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT19371.4423900335837845No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT18331.3649462733913664No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC17831.327713696375781No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA16621.2376108599980638No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA16251.2100587530065305No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC15041.1199559166288136No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA14531.0819786880729163No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA14451.0760214757504227No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA14291.0641070511054351No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC13811.0283637771704732No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT11760.8757102114065723No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA10970.8168827397219471No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT10650.7930538904319724No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC10610.7900752842707255No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA10600.7893306327304138No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA10020.7461408433923346No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA9960.7416729341504643No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA8850.6590166131758644No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA8260.6150821722974734No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC8250.6143375207571616No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA8190.6098696115152914No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCAAAAACATATCCTAAACCA7360.5480635336694194No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT6410.47732163733980687No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA6370.47434303117855997No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA6360.47359837963824825No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT6190.4609393034529492No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC6060.45125883342889694No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA6050.4505141818885852No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5770.42966393875985737No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA5700.42445137797767535No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA5530.41179230179237625No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA5010.3730704216961673No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT4700.34998622394650425No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4560.3395611023821403No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC4330.32243411695497093No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA4290.3194555107937241No Hit
AAAATTAACCTAAAAATCCCCACAACCATAACTCTACCTATATTCCCACA4090.3045624799874898No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA3990.2971159645843728No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3960.2948820099634376No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3930.2926480553425025No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT3780.2814782822378268No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA3740.27849967607658No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3310.24647965984317638No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC3290.24499035676255299No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA3200.23828849289974757No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA3130.23307593211756558No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3000.22339546209351335No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCGATCCCCTTCCTCCCAT2860.21297034052914937No Hit
AACTAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCG2840.21148103744852595No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA2710.20180056742447372No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2640.19658800664229173No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2630.19584335510198003No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC2610.1943540520213566No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2600.19360940048104489No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2580.1921200974004215No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT2580.1921200974004215No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA2560.19063079431979804No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTG2540.18914149123917462No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG2540.18914149123917462No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA2480.18467358199730435No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2460.18318427891668093No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA2440.1816949758360575No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA2410.17946102121512236No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2130.15861077808639448No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC1990.14818565652203053No Hit
ATATAATACCCACCTTCCAAACCACAAACCCACAACCCAATCCTAAAAAC1960.14595170190109538No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA1850.13776053495766657No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA1790.1332926257157963No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC1790.1332926257157963No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1770.13180332263517286No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC1750.13031401955454944No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAACCCCCCCAACAA1730.12882471647392602No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1720.12808006493361432No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA1690.12584611031267917No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT1650.12286750415143233No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG1570.11691029182893865No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT1550.11542098874831523No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA1530.1139316856676918No Hit
AAAATTAACCTAAAAATCCCCACAAACAAAAAAAACAAATATCTAAAACC1510.11244238258706837No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC1500.11169773104675668No Hit
CCCTTTAAACTATCCCACCTTTCTAAACTAAACCAATATACATCTTAAAT1390.10350656410332784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)