FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164933
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT63153.828827463272965No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA63023.820945474829173No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA57623.4935398010101073No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG54963.332262191314049No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA46452.8162951016473357No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG45752.7738536254115305No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT43602.643497662687273No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT40012.425833520277931No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT39582.3997623277330797No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT39372.387029884862338No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC37102.2493982404976567No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT35622.1596648335990976No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA34342.0820575627679117No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG31431.9056222829876375No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC31141.8880393856899467No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA28321.71706086713999No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA27781.6843202997580835No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA27511.6679500160671303No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCTATTCGAA26681.617626551387533No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC26101.582460756792152No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT25321.535168826129398No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACGTCTAAAA23471.4230020675061996No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA21891.327205592573954No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA21641.3120479224897383No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTGCCTACCG21131.2811262755179376No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA20011.2132199135406498No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC19041.1544081536138917No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA17911.0858954848332352No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATCAAACAAAACGTCTAAAA17371.0531549174513286No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT16611.007075600395312No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA13120.7954745260196565No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA10400.6305590755033863No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9400.5699283951665222No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC9080.5505265774587257No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT7900.4789823746612261No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7860.4765571474477515No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT7430.45048595490289994No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC6550.39713095620645955No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT6390.38743004735256137No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCGATCCTAAAAAC6010.364390388824553No Hit
AACTAATCTAAACCTCCTAACCTCAAGCGATCTATCTACTTCTACCTCCG5680.34438226431338786No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT5140.31164169693148125No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT4750.2879957316001043No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4610.27950743635294334No Hit
AACAACAATATTTCCTAATCCTCCCAAAATCCTAAAATTCTCCTAACCTG4590.27829482274620604No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCTATTCTAA4390.26616868667883326No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA3960.24009749413398165No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3940.2388848805272444No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAACTCTA3890.2358533465104012No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC3760.22797135806660887No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA3700.22433351724639702No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC3260.19765601789817683No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT3050.18492357502743537No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA3030.1837109614206981No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2980.1806794274038549No Hit
TCTCAAACCTACTACCTAAAAACCTCATCTACATAACAAAAACCTTAACC2750.16673437092637616No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA2600.15763976887584658No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA2580.15642715526910927No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2550.15460823485900335No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA2510.15218300764552878No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2470.1497577804320542No Hit
CAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT2450.14854516682531693No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2440.1479388600219483No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2310.14005687157815597No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT2150.13035596272425773No Hit
CTCCCAACCCCCACTCCTAAACACACTATTCCAAAACATATCCTAAACCA2140.1297496559208891No Hit
CACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA2060.12489920149393996No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC2030.12308028108383405No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCGAACACTA2030.12308028108383405No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCTATTCGAA2010.12186766747709675No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA1960.11883613346025355No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACTTCTAAAA1930.11701721305014763No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA1900.11519829264004171No Hit
CCCTTTAAACTATCCCACCTTTCTAAACTAAACCAATATACATCTTAAAT1890.11459198583667309No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA1860.11277306542656715No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG1720.10428477017940618No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA1710.10367846337603753No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC1700.10307215657266891No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCTATT1660.10064692935919434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)