FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155901
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG58523.7536641843221017No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA47393.039749584672324No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA44872.8781085432421856No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT40482.596519586147619No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA39082.5067190075753203No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA37702.418201294411197No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA37122.3809981975741015No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG35022.2462973297156528No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT31131.996780007825479No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC30991.987799949968249No Hit
CTCCCAACCCCCACTCCTAAACACGCCATTCCAAAACATATCCTAAACCA29111.8672106015997332No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA29001.8601548418547669No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT28301.8152545525686175No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA26931.7273782721085817No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT24601.5779244520561126No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC23681.5189126432800304No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA23261.4919724697083405No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG20791.3335385917986415No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT20451.3117298798596546No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT20381.3072398509310394No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC20001.2828654081757014No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT19741.266188157869417No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA18841.2084592145015105No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT18541.1892162333788752No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA17471.120582934041475No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA17031.0923598950616096No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT16921.0853041353166433No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA16561.0622125579694806No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC15520.9955035567443442No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA14830.9512447001622826No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC14120.905702978172045No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC13790.884535698937146No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT13740.8813285354167067No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG12470.7998665819975498No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA12030.7716435430176842No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA11030.7075002726088992No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT8870.5689508085259235No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA8820.5657436450054842No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA8390.5381620387297067No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7880.5054489708212263No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT7660.49133745133129353No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA7260.46568014316777956No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA7100.455417219902374No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7000.4490028928614954No Hit
AACTAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCA6980.44772002745331974No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA6970.44707859474923184No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA6860.44002283500426553No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT6770.4342499406674749No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACGCCGAA6320.40538546898352157No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5970.3829353243404468No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC5910.37908672811591976No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC5840.37459669918730476No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA5770.3701066702586898No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA5650.3624094778096356No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT5440.34893939102379073No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACGCCGAAC5300.33995933316656085No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA5280.33867646775838517No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT4350.279023226278215No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCAAAAAA4160.2668360049005459No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACGTCTAAAA4070.2610631105637552No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4030.25849737974740383No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA3890.2495173218901739No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT3850.2469515910738225No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3700.23733010051250475No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT3640.23348150428797762No Hit
TCTCTTTACATTTAATCAAAAACCCAAACCTTACTACTAAAATAACCCCA3590.2302743407675384No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT3580.22963290806345052No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC3450.22129428291030848No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCCGAAAAA3440.2206528502062206No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT3410.21872855209395708No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3250.20846562882855146No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT3240.20782419612446362No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3230.20718276342037578No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2990.19178837852226732No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACGCCGA2780.17831829173642247No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA2700.17318683010371966No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC2520.16164104143013835No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA2390.15330241627699628No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2380.15266098357290844No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC2340.15009525275655705No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTGTCCACCTCCTAAAACA2330.1494538200524692No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA2310.14817095464429347No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCAAACC2270.14560522382794208No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2140.13726659867480004No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC2050.1314937043380094No Hit
TCTCTTTACATTTAATCAAAAACCCAAACCTTACCACTAAAATAACCCCA2030.13021083892983368No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1980.12700367540939442No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCAA1950.12507937729713087No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT1940.12443794459304303No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT1920.12315507918486732No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCCGAAAAA1850.11866505025625237No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1830.11738218484807665No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC1740.111609290511286No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACGCCGAACC1690.10840212699084677No Hit
CCCAAAATCCTAAAATTCTCCTAACCACGAAAACTCCCCACAAAAACAAA1650.10583639617449535No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1590.10198779994996825No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1580.10134636724588039No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA1580.10134636724588039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)