FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94712
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG39914.213827181349776No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA30713.2424613565334908No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA28563.0154573866035985No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA26862.835965875496241No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT24622.599459413801841No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA24082.5424444632147982No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA23122.441084551060056No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT22452.3703437790353914No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG22172.3407804713235913No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT18731.977574119435763No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC18731.977574119435763No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG18281.9300616606132275No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT17251.8213109215305348No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC15591.6460427400962918No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA15361.6217585944758848No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT15301.6154235999662134No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC14941.577413632908185No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA14561.537292001013599No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA14131.4918912070276207No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA12411.3102880310837064No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT12261.2944505448095278No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT12171.2849480530450206No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT11821.2479939184052709No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCAAA11701.235323929385928No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA11551.2194864431117494No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC11091.170918151870935No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA9701.0241574457302136No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA9330.9850916462539065No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA9310.9829799814173494No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA8820.9312441929216995No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT8130.8583917560604781No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC7960.8404426049497423No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT7950.8393867725314638No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA7550.7971534758003209No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC7380.7792043246895853No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA6550.6915702339724639No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA6130.6472252724047639No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA6060.6398344454768139No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC5340.5638145113607569No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5310.5606470141059211No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA5140.5426978629951854No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA5010.528972041557564No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT4990.5268603767210068No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC4800.506799560773714No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA4690.4951854041726498No Hit
ACCAAACAACCAAACTAAACAACGCCAAAAACGAAAAACCCAAATATCCT4460.4709012585522426No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC4330.45717543711462116No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA4000.42233296731142833No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG3200.33786637384914264No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC3030.31991722273840695No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA3030.31991722273840695No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT2920.3083030661373427No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA2720.28718641777177123No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA2690.28401892051693556No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2650.2797955908438213No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA2420.2555114452234141No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT2370.2502322831320213No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT2360.24917645071374273No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTCAAATCCTCC2210.23333896443956414No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCCGAAAAA2200.2322831320212856No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2090.22066897542022132No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCAA2070.21855731058366418No Hit
ATATCTCCTTACATCCCAACCTCGTCTATACAACAAAAAACCAAAAACTT2010.21222231607399272No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA1860.19638482979981417No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA1850.1953289973815356No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC1690.17843567868907848No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA1680.1773798462707999No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT1680.1773798462707999No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA1620.1710448517611285No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT1590.16787735450629276No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT1530.16154235999662134No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT1440.15203986823211418No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA1430.15098403581383563No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC1410.14887237097727848No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1380.14570487372244278No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA1340.1414815440493285No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1330.14042571163104992No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT1300.13725821437621422No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCAAA1280.13514654953965705No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCGATCCCCTTCCTCCCAT1260.13303488470309993No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA1220.12881155502998565No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA1200.1266998901934285No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTGCCTCCG1190.12564405777514995No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1110.11719739842892136No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA1110.11719739842892136No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1040.10980657150097137No Hit
CCCTTTAAACTATCCCACCTTTCTAAACTAAACCAATATACATCTTAAAT1010.10663907424613564No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT990.10452740940957851No Hit
ATATAATACCCACCTTCCAAACCACAAACCCTCAACCCAATCCTAAAAAC990.10452740940957851No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA980.10347157699129993No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT970.10241574457302137No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT970.10241574457302137No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC960.1013599121547428No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG950.10030407973646424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)