FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130407
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG89046.827854332972922No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCG63404.861702209237234No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG61944.749745029024516No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG58764.505893088561197No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG54764.199161087978406No Hit
GGTTTGGAAGGTGGGTATTATATTTTGTTTTGTTTTTGAAGTTTAGTTTT51573.9545423175136305No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT48693.7336952770940206No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGCGTTGTTGGTGTTGTATTTTG48283.7022552470342847No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG45453.485242356621961No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG44113.3824871364267253No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA40423.099526865889101No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA40353.0941590558789023No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT36882.828069045373331No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT31212.3932764345472255No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT29492.2613816742966253No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTCGGGCGTAGGGGATAA28612.1939006341684113No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA20311.5574317329591203No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT19841.5213907228906425No Hit
GGTTGGAGTTTTAGGTGTATTTGGGTGTAGGTTTTTTTGAGGAAATTAAG17771.3626569125890482No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTCGGGCGTAGGGGATAAG13521.0367541619698328No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGCGTGGT12650.9700399518430759No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG10760.8251090815677072No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT10640.8159071215502235No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG10260.7867675814948584No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG9170.7031831113360479No Hit
GGTAGGAGGGGTTGTATAAGGTTTTGGAGAGGAGGTTTTTAGATTAGACC7920.6073293611539258No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTGTTTGTTTGTG7860.6027283811451839No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT7830.600427891140813No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT7290.5590190710621362No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTTCGGGCGTAGGGGATA6920.530646361008228No Hit
GAAGTTGTGGAATTTAGGTAGGAGTGGGGGTTGGGAGAGACCAAGTCTCT6910.5298795310067711No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT6030.4623984908785571No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTAGGGTTTCGTTTTCGTTTAGT5310.4071867307736548No Hit
AAAATATTAGAGGAAGGAGAGGTTGGGATGTAAGGAGATATAGACCAAGT5230.401052090761999No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTAAAGGTATAGTTTTAGGAGGT5220.40028526076054205No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT5210.399518430759085No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATCTTTTATGAATTTTTTTTTTAT4700.3604101006847792No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGCGTGGTAGG3540.27145782051576983No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG3450.2645563505026571No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG3300.25305390048080245No Hit
AAAATATTAGAGGAAGGAGGGGTTGGGATGTAAGGAGATATAGACCAAGT3230.24768609047060358No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA2930.22468119042689427No Hit
TGGGTTTTAGATATTTGTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAGG2680.2055104403904698No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTATT2590.19860897037735706No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG2410.18480603035113144No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGC2270.17407041033073378No Hit
GGAAGGTATGTGGGGTTTAGTTTTAGTTTGGTATCGTCGTTTTTTTTTAG2210.1694694303219919No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.133428420253514No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTTAGGGTTTCGTTTTCGTTTA1740.133428420253514No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTATT1730.132661590252057No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTGGGTGTAGGGGATAAGA1630.12499329023748726No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTAAAGAGT1610.1234596302345733No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTCTAGTAGATTGGAGGA1540.11809182022437445No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTTA1510.11579133022000351No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATCTTTTATGAATTTTTTTTTATT1460.11195718021271865No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTAGG1440.1104235202098047No Hit
GAAGTTGTGGAATTTAGGTAGGAATGGGGGTTGGGAGAGACCAAGTCTCT1410.10812303020543376No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGATGGTGAGG1400.10735620020397678No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT1330.10198839019377794No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTTTCGGGCGTAGGGGAT1320.10122156019232098No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGAGGTTGTGGATGATACGTTGGTT1310.100454730190864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)