FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139460
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG63694.56690090348487No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA47273.389502366269898No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA41893.003728667718342No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA40482.9026244084325254No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA39832.856016061953248No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT39052.800086046178116No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA35362.5354940484726805No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT32882.3576652803671303No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG31692.272336153735838No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT29912.1447009895310485No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA29442.1109995697691097No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT25961.861465653233902No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT24301.7424351068406712No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC23511.685788039581242No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC22411.6069123763086188No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA22291.5983077584970602No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA21861.5674745446723075No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT20401.4627850279650079No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC19301.383909364692385No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA18791.3473397389932595No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC17411.2483866341603327No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA17051.2225727807256561No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG16861.2089488025240211No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT16811.2053635451025384No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA15971.1451312204216262No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA15651.122185572924136No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT14731.0562168363688513No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA13290.9529614226301448No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA11520.8260433099096515No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT11300.810268177255127No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT10490.7521870070271046No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA9520.6826330130503371No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT9310.667574931880109No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA8490.60877671016779No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC8410.6030402982934174No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA7630.5471102825182849No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA7240.5191452746307185No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7150.5126918112720493No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT6770.48544385486877956No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC6730.48257564893159327No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCAAA6730.48257564893159327No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA6350.45532769252832356No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC6310.45245948659113727No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA6230.44672307471676465No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG6200.44457192026387493No Hit
AACTAATCTAAACCTCCTAACCTCAAGCGATCTATCTACTTCTACCTCCG6110.4381184569052058No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC5850.41947511831349493No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5560.39868062526889436No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA5420.3886419044887423No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA4860.34848702136813425No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA4850.3477699698838376No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCGTAACGAAAAATA4560.32697547683923706No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATGCATCTTAAAT4410.31621970457478843No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG4240.3040298293417467No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3790.271762512548401No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC3790.271762512548401No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA3780.2710454610641044No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCAAAC3780.2710454610641044No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT3680.2638749462211387No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3540.25383622544098666No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA3330.23877814427075866No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATCA3070.22013480567904775No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2860.20507672450881972No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA2800.20077441560304032No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2780.19934031263444718No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACCACCGAAACCCATTCGAA2690.19288684927577798No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC2630.18858454036999858No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT2630.18858454036999858No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA2590.18571633443281227No Hit
ACACTCTAAAATTTCCCCTAAAAAACTATTCAAACCATAACAAAAAATAA2550.18284812849562598No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC2420.17352645919977056No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2310.16563889287250824No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2150.15416606912376307No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2140.1534490176394665No Hit
TAATACCAACTATACCACTCAACTCCTAAAAATTACCTTCCAAAAACTCT2140.1534490176394665No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC2060.14771260576509393No Hit
ATATAATACCCACCTTCCAAACCACGAACCCTCAACCCAATCCTAAAAAC2050.14699555428079736No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA2020.14484439982790764No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCAA2000.1434102968593145No Hit
AAATAATAACATTAAATACTCCTCCCAAATACTCCTCCCTCCTCCTCCTC1920.1376738849849419No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA1890.1355227305320522No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT1780.1276351642047899No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC1760.12620106123619676No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT1710.1226158038147139No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT1710.1226158038147139No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1700.12189875233041732No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT1690.12118170084612075No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA1640.11759644342463789No Hit
AACTAATCTAAACCTCCTAACCTCAATCGATCTATCTACTTCTACCTCCG1620.11616234045604475No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA1600.1147282374874516No Hit
CCAAAAAAACCAACAAAAAAACTAAATGAAACCGAAAACTTCTCCATCTT1590.11401118600315502No Hit
ACCAAACAACCAAACTAAACAACGCTGAAAACAAAAAACCCAAATATCCT1580.11329413451885845No Hit
ACTAAATAAAAACTTACCCAACCCCTCTCTACCAATCCCCTTCCTCCCAT1580.11329413451885845No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT1580.11329413451885845No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA1560.1118600315502653No Hit
AACCCAATCTAAAACCAAAAACCTACTCATCCAATATAAAAAAAACAACT1550.11114298006596873No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA1550.11114298006596873No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCAAACC1540.11042592858167216No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA1520.10899182561307902No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT1500.10755772264448588No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCTAAAAAA1490.10684067116018932No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCTAAAAAA1490.10684067116018932No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT1460.10468951670729958No Hit
CCCAAAATCCTAAAATTCTCCTAACCTCAAAAACTCCCCACAAAAACAAA1450.103972465223003No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA1400.10038720780152016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)