FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150548
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA48013.189016127746632No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA42922.850917979647687No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA42752.8396259000451685No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT41742.772537662406674No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG41042.7260408640433615No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA37692.503520471876079No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT37452.4875787124372293No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT34022.259744400456997No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA33842.2477880808778594No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT32872.183356803145841No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG32692.171400483566703No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC31632.1009910460451153No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT29611.9668145707681273No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT29381.9515370513058956No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA27621.8346308154209952No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC27551.829981135584664No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA24951.6572787416637882No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA23621.5689348247734942No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA21871.4526928288652123No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA21461.4254589898238434No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC21101.4015463506655685No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC20961.3922469909929058No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA20901.3882615511331935No Hit
ATATAATACCCACCTTCCAAACCACGAATCCGCAACCCAATCCTAAAAAC19391.2879613146637616No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA18101.2022743576799426No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT17401.1557775593166297No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC16841.1185801206259798No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT16311.0833754018651858No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA16111.0700906023328107No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT15711.0435210032680606No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCGAA15221.0109732444137418No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA14530.965140686027048No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14160.9405638068921539No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA12850.8535483699550974No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA12770.8482344501421473No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAATTCCACTAACCCCGAAC12510.8309642107500598No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG10940.7266785344209156No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA10200.6775247761511278No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA9410.6250498179982464No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG9230.6130934984191089No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA8500.5646039801259399No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAACAACCGTCCTAACCT7810.518771421739246No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7710.5121290219730584No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT7190.4775885431888833No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG6600.4383983845683769No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA6100.4051863857374392No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAATTCCACTAACCCCGAACC5630.3739671068363578No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4980.3307915083561389No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAAATTCCACTAACCCCGA4880.3241491085899514No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT4340.2882801498525387No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT3930.26104631081116986No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCTCATAATACAACCTAAATCC3830.25440391104498233No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC3690.2451045513723198No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3560.23646943167627602No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCTAA3450.22916279193346975No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT3360.22318463214390094No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA3340.22185615219066346No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT3190.21189255254138217No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3100.20591439275181336No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT3020.20060047293886338No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT2970.19727927305576962No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCTAAAACTTCTCCATCTT2660.17668783378058825No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA2580.17137391396763824No Hit
CAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT2570.17070967399101947No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT2540.16871695406116322No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA2520.1673884741079257No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2510.16672423413130696No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACTAAACCCCGAAAAA2490.16539575417806945No Hit
CACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG2260.15011823471583816No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCTAAACCCATTCGAA2230.14812551478598188No Hit
CCTAAAACAAACTAATACCACCCACTAAACTAAAACGAAACCCCGAAAAA2220.14746127480936314No Hit
CAAAACTAACTCTAAAATTTCCTTTTTCAATCTTAACCTAATAACCAACT2180.14480431490288811No Hit
AAAAACTAACACACACTTATACCCCCTAAACAACCATAAATACACAAAAA2050.13616919520684434No Hit
CCCTTTAAACTATCCCACCTTTCTAAACCAAACCGATATATTTCTTAAAC2020.1341764752769881No Hit
CCCTTTAAACTATCCCACCTTTCTAAACTAAACCAATATACATCTTAAAT2010.13351223530036932No Hit
ACTAAATAAAAACTTACCCAACCCCTCTCTACCAATCCCCTTCCTCCCAT1970.1308552753938943No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACAACCCAAACACTA1960.13019103541727556No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1900.12620559555756303No Hit
AACTAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCG1900.12620559555756303No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAAACTCTACTAACCCATCTCC1890.12554135558094429No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC1860.12354863565108803No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC1800.1195631957913755No Hit
ATATAATACCCACCTTCCAAACCACTAATCCGCAACCCAATCCTAAAAAC1790.11889895581475675No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA1750.11624199590828176No Hit
ATATAATACCCACCTTCCAAACCACTAACCCGCAACCCAATCCTAAAAAC1750.11624199590828176No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1730.11491351595504425No Hit
AAATAATAACATTAAATACTCCTCCCAATACTCCTCCTCCTCCTCCTACT1720.11424927597842548No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA1720.11424927597842548No Hit
ATATAATACCCACCTTCCAAACCACGAATCCTCAACCCAATCCTAAAAAC1720.11424927597842548No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT1720.11424927597842548No Hit
CTATAATACCCACCTTCCAAACCACGAATCCGCAACCCAATCCTAAAAAC1680.11159231607195047No Hit
AACTAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCG1670.11092807609533173No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCTAA1670.11092807609533173No Hit
CAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC1660.11026383611871296No Hit
CCTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT1660.11026383611871296No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCAAC1630.10827111618885671No Hit
CACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC1600.10627839625900046No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC1590.10561415628238172No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA1590.10561415628238172No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCTCCACGACCCAAACACTA1570.10428567632914419No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT1540.10229295639928794No Hit
CCCTAAAAACAAACTAAAACTATAAAAAAAAAAATTCCACTAACCCCTAA1520.10096447644605043No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA1510.10030023646943169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)